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@ARTICLE{Chu:1050648,
      author       = {Chu, Ci and Vargas, Carolyn and Barbosa, Maria Carolina and
                      Sommerhage, Simon and Rechberger, Gerald N. and Pahovnik,
                      David and Žagar, Ema and Schröder, Gunnar F. and Keller,
                      Sandro and Etzkorn, Manuel},
      title        = {{C}apturing {G} protein-coupled receptors into native
                      lipid-bilayer nanodiscs using new diisobutylene/maleic acid
                      ({DIBMA}) copolymers},
      journal      = {Methods},
      volume       = {244},
      issn         = {1046-2023},
      address      = {Orlando, Fla.},
      publisher    = {Academic Press},
      reportid     = {FZJ-2026-00400},
      pages        = {55 - 64},
      year         = {2025},
      abstract     = {Many membrane proteins, including G protein-coupled
                      receptors (GPCRs), are susceptible to denaturation when
                      extracted from their native membrane by detergents.
                      Therefore, alternative methods have been developed,
                      including amphiphilic copolymers that enable the direct
                      extraction of functional membrane proteins along with their
                      surrounding lipids. Among these amphiphilic copolymers,
                      styrene/maleic acid (SMA) and diisobutylene/maleic acid
                      (DIBMA) polymers have been extensively studied. Despite
                      their many benefits, SMA and DIBMA polymers also have
                      considerable drawbacks limiting their applications. Herein,
                      we describe a series of new amphiphilic copolymers derived
                      from DIBMA via partial amidation of the carboxylate pendant
                      groups with various biocompatible amines. We characterize
                      the new polymer’s nanodisc-forming properties and ability
                      to extract the melanocortin 4 receptor (MC4R), a
                      prototypical class A GPCR. While each new DIBMA variant
                      displays features that may be favorable for selected
                      applications, we identified a PEGylated DIBMA variant called
                      mPEG4-DIBMA as particularly promising. In the tested system
                      mPEG4-DIBMA abolishes unspecific interactions and
                      outperforms other polymers by achieving higher extraction
                      efficiencies of MC4R from Sf9 insect cell membranes. The new
                      nanodisc-forming polymer combines two key advantages that
                      are crucial for investigating GPCRs in a well-defined but
                      still native lipid-bilayer environment, thus paving the way
                      for manifold future applications.},
      cin          = {ER-C-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {5352 - Understanding the Functionality of Soft Matter and
                      Biomolecular Systems (POF4-535) / 5241 - Molecular
                      Information Processing in Cellular Systems (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1016/j.ymeth.2025.08.013},
      url          = {https://juser.fz-juelich.de/record/1050648},
}