% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Sachse:1050650,
      author       = {Sachse, Carsten},
      title        = {{C}ryogenic {STEM} of thick biological specimens},
      journal      = {Nature methods},
      volume       = {22},
      number       = {10},
      issn         = {1548-7091},
      address      = {London [u.a.]},
      publisher    = {Nature Publishing Group},
      reportid     = {FZJ-2026-00402},
      pages        = {2015 - 2016},
      year         = {2025},
      abstract     = {A cryogenic scanning transmission electron microscopy
                      (STEM) approach for analyzing thick biological specimens
                      expands the reach of cryo-electron microscopy.In the past
                      decade, structure determination and visualization of
                      biological macromolecules by cryogenic electron microscopy
                      (cryo-EM) has become one of the most popular tools in
                      structural biology1. The power of cryo-EM has relied on the
                      capabilities of transmission electron microscopy (TEM),
                      which involves image formation in the microscope through
                      electromagnetic lenses followed by comprehensive
                      single-particle image processing. A few thousand
                      well-defined particles can be sufficient to generate a
                      resolution allowing reliable atomic model building. Despite
                      the power of the established cryo-TEM approach, biological
                      specimens can be too small, too heterogeneous or too thick
                      and thus fall short of the commonly achieved resolutions.
                      Moreover, determining biological structures at this
                      resolution within the native cellular environment has only
                      been possible in thin focused ion beam milled sections of
                      approximately 100 nm thickness for very large and abundant
                      macromolecular complexes such as the ribosome. In this
                      issue, Yu et al.2 propose an alternative approach for
                      imaging thick specimens that is based on cryogenic scanning
                      transmission electron microscopy (STEM) followed by image
                      processing, a method they call tilt-corrected bright-field
                      STEM (tcBF-STEM).},
      cin          = {ER-C-3},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524) / 5352 - Understanding the Functionality of Soft
                      Matter and Biomolecular Systems (POF4-535)},
      pid          = {G:(DE-HGF)POF4-5241 / G:(DE-HGF)POF4-5352},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1038/s41592-025-02818-9},
      url          = {https://juser.fz-juelich.de/record/1050650},
}