001052075 001__ 1052075
001052075 005__ 20260120203627.0
001052075 0247_ $$2doi$$a10.1016/j.crgsc.2025.100448
001052075 0247_ $$2datacite_doi$$a10.34734/FZJ-2026-00744
001052075 037__ $$aFZJ-2026-00744
001052075 082__ $$a540
001052075 1001_ $$00009-0007-6917-9730$$aEberz, Jörg$$b0
001052075 245__ $$aPhase separation behaviour during direct solvent extraction of Corynebacterium glutamicum fermentation broth – Systematic study of crud suppression
001052075 260__ $$aAmsterdam$$bElsevier$$c2025
001052075 3367_ $$2DRIVER$$aarticle
001052075 3367_ $$2DataCite$$aOutput Types/Journal article
001052075 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1768923370_1107
001052075 3367_ $$2BibTeX$$aARTICLE
001052075 3367_ $$2ORCID$$aJOURNAL_ARTICLE
001052075 3367_ $$00$$2EndNote$$aJournal Article
001052075 500__ $$aFunding: Systematische Untersuchung der Flüssig-flüssig-Extraktion von Fermentationsbrühen mit biologischen Feststoffen – Grant No. 01IF22343 N”, by the Federal Ministry of Economic Affairs and Climate Action (BMWK) within the framework of the “Industrielle Gemeinschaftsforschung" program based on a resolution of the German Bundestag
001052075 520__ $$aThe economic competitiveness of bio-based production processes is often hindered by the high costs associated with downstream processing, compared to fossil-based methods. Liquid-liquid extraction is a widely used technique for aqueous fermentation systems and offers significant cost-saving potential, especially if extraction could be performed directly from the fermentation broth without prior cell separation. However, this is often hindered by the formation of "crud" — a deposit or emulsion at the interface between two partially settled phases. This study investigates the liquid-liquid phase separation of Corynebacterium glutamicum DM 1933 fermentation broths using five different solvents. We systematically examined the impact of cell surface properties, modified through nutrient concentration, on crud formation. In addition, the variation in salt concentration and pH after fermentation was analysed. Our findings show that the present nutrient concentration influences the cell surface properties and, consequently, crud formation. A more hydrophilic cell surface was present at a lower phosphate concentration, whereas a more hydrophobic cell surface was measured for a lower nitrogen and iron concentration. With a more hydrophobic cell surface, the fermentation broth showed a large crud phase, while a decrease in crud formation could be seen for fermentation broths with a more hydrophilic cell surface. Furthermore, the crud formation is influenced by the pH, cell and salt concentration and strongly by the used solvent.
001052075 536__ $$0G:(DE-HGF)POF4-2172$$a2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217)$$cPOF4-217$$fPOF IV$$x0
001052075 588__ $$aDataset connected to CrossRef, Journals: juser.fz-juelich.de
001052075 7001_ $$00009-0008-2550-8958$$aStrehl, Lara$$b1
001052075 7001_ $$00000-0002-2819-0358$$aMann, Marcel$$b2
001052075 7001_ $$0P:(DE-Juel1)194474$$aJupke, Andreas$$b3$$eCorresponding author
001052075 7001_ $$00009-0007-4381-0088$$aMagnus, Jørgen Barsett$$b4
001052075 773__ $$0PERI:(DE-600)3023948-5$$a10.1016/j.crgsc.2025.100448$$gVol. 10, p. 100448 -$$p100448 -$$tCurrent research in green and sustainable chemistry$$v10$$x2666-0865$$y2025
001052075 8564_ $$uhttps://juser.fz-juelich.de/record/1052075/files/1-s2.0-S2666086525000049-main.pdf$$yOpenAccess
001052075 909CO $$ooai:juser.fz-juelich.de:1052075$$popenaire$$popen_access$$pVDB$$pdriver$$pdnbdelivery
001052075 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)194474$$aForschungszentrum Jülich$$b3$$kFZJ
001052075 9131_ $$0G:(DE-HGF)POF4-217$$1G:(DE-HGF)POF4-210$$2G:(DE-HGF)POF4-200$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-2172$$aDE-HGF$$bForschungsbereich Erde und Umwelt$$lErde im Wandel – Unsere Zukunft nachhaltig gestalten$$vFür eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten$$x0
001052075 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-18
001052075 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
001052075 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2023-05-02T08:54:38Z
001052075 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2023-05-02T08:54:38Z
001052075 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
001052075 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Anonymous peer review$$d2023-05-02T08:54:38Z
001052075 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2024-12-18
001052075 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2024-12-18
001052075 9201_ $$0I:(DE-Juel1)IBG-2-20101118$$kIBG-2$$lPflanzenwissenschaften$$x0
001052075 980__ $$ajournal
001052075 980__ $$aVDB
001052075 980__ $$aUNRESTRICTED
001052075 980__ $$aI:(DE-Juel1)IBG-2-20101118
001052075 9801_ $$aFullTexts