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| 001 | 10586 | ||
| 005 | 20200402205851.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:21386436 |
| 024 | 7 | _ | |2 DOI |a 10.1088/0953-8984/22/19/194109 |
| 024 | 7 | _ | |2 WOS |a WOS:000277033100012 |
| 037 | _ | _ | |a PreJuSER-10586 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 530 |
| 084 | _ | _ | |2 WoS |a Physics, Condensed Matter |
| 100 | 1 | _ | |a Kirchenbüchler, D. |b 0 |u FZJ |0 P:(DE-Juel1)VDB84090 |
| 245 | _ | _ | |a Substrate, focal adhesion and actin filaments: A mechanical unit with a weak spot for mechanosensitive proteins |
| 260 | _ | _ | |a Bristol |b IOP Publ. |c 2010 |
| 300 | _ | _ | |a 194109 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Journal of Physics: Condensed Matter |x 0953-8984 |0 3703 |v 22 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a Mechanosensing is a vital prerequisite for dynamic remodeling of focal adhesions and cytoskeletal structures upon substrate deformation. For example, tissue formation, directed cell orientation or cell differentiation are regulated by such mechanosensing processes. Focal adhesions and the actin cytoskeleton are believed to be involved in these processes, but where mechanosensing molecules are located and how elastic substrate, focal adhesions and the cytoskeleton couple with each other upon substrate deformation still remains obscure. To approach these questions we have developed a sensitive method to apply defined spatially decaying deformation fields to cells cultivated on ultrasoft elastic substrates and to accurately quantify the resulting displacements of the actin cytoskeleton, focal adhesions, as well as the substrate. Displacement fields were recorded in live cell microscopy by tracking either signals from fluorescent proteins or marker particles in the substrate. As model cell type we used myofibroblasts. These cells are characterized by highly stable adhesion and force generating structures but are still able to detect mechanical signals with high sensitivity. We found a rigid connection between substrate and focal adhesions. Furthermore, stress fibers were found to be barely extendable almost over their whole lengths. Plastic deformation took place only at the very ends of actin filaments close to focal adhesions. As a result, this area became elongated without extension of existing actin filaments by polymerization. Both ends of the stress fibers were mechanically coupled with detectable plastic deformations on either site. Interestingly, traction force dependent substrate deformation fields remained mostly unaffected even when stress fiber elongations were released. These data argue for a location of mechanosensing proteins at the ends of actin stress fibers and describe, except for these domains, the whole system to be relatively rigid for tensile strain with a mechanical coupling between the front and rear end of a cell. |
| 536 | _ | _ | |a BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung |c P45 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK505 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Actin Cytoskeleton: physiology |
| 650 | _ | 2 | |2 MeSH |a Animals |
| 650 | _ | 2 | |2 MeSH |a Cell Adhesion: physiology |
| 650 | _ | 2 | |2 MeSH |a Cells, Cultured |
| 650 | _ | 2 | |2 MeSH |a Fibroblasts: physiology |
| 650 | _ | 2 | |2 MeSH |a Intracellular Signaling Peptides and Proteins: physiology |
| 650 | _ | 2 | |2 MeSH |a Mechanotransduction, Cellular: physiology |
| 650 | _ | 2 | |2 MeSH |a Rats |
| 650 | _ | 2 | |2 MeSH |a Rats, Wistar |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Intracellular Signaling Peptides and Proteins |
| 650 | _ | 7 | |a J |2 WoSType |
| 700 | 1 | _ | |a Born, S. |b 1 |u FZJ |0 P:(DE-Juel1)161241 |
| 700 | 1 | _ | |a Kirchgeßner, N. |b 2 |u FZJ |0 P:(DE-Juel1)VDB8902 |
| 700 | 1 | _ | |a Houben, S. |b 3 |u FZJ |0 P:(DE-Juel1)VDB87855 |
| 700 | 1 | _ | |a Hoffmann, B. |b 4 |u FZJ |0 P:(DE-Juel1)VDB27696 |
| 700 | 1 | _ | |a Merkel, R. |b 5 |u FZJ |0 P:(DE-Juel1)128833 |
| 773 | _ | _ | |a 10.1088/0953-8984/22/19/194109 |g Vol. 22, p. 194109 |p 194109 |q 22<194109 |0 PERI:(DE-600)1472968-4 |t Journal of physics / Condensed matter |v 22 |y 2010 |x 0953-8984 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1088/0953-8984/22/19/194109 |
| 909 | C | O | |o oai:juser.fz-juelich.de:10586 |p VDB |
| 913 | 1 | _ | |k P45 |v BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung |l Biologische Informationsverarbeitung |b Schlüsseltechnologien |0 G:(DE-Juel1)FUEK505 |x 0 |
| 913 | 2 | _ | |a DE-HGF |b Key Technologies |l BioSoft Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences |1 G:(DE-HGF)POF3-550 |0 G:(DE-HGF)POF3-552 |2 G:(DE-HGF)POF3-500 |v Engineering Cell Function |x 0 |
| 914 | 1 | _ | |y 2010 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k IBN-4 |l Biomechanik |d 31.12.2010 |g IBN |0 I:(DE-Juel1)VDB802 |x 0 |
| 970 | _ | _ | |a VDB:(DE-Juel1)120977 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-7-20110106 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-2-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-7-20110106 |
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