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000010587 084__ $$2WoS$$aBiochemical Research Methods
000010587 084__ $$2WoS$$aBiochemistry & Molecular Biology
000010587 084__ $$2WoS$$aChemistry, Multidisciplinary
000010587 084__ $$2WoS$$aChemistry, Organic
000010587 1001_ $$0P:(DE-Juel1)128805$$aCsiszar, A.$$b0$$uFZJ
000010587 245__ $$aNovel Fusogenic Liposomes for Fluorescent Cell labeling and Membrane Modification
000010587 260__ $$aColumbus, Ohio$$bAmerican Chemical Society$$c2010
000010587 300__ $$a537 - 543
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000010587 440_0 $$022602$$aBioconjugate Chemistry$$v21$$x1043-1802$$y3
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000010587 520__ $$aEfficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications. The fusion efficiency of these liposome systems was tested on single cells in culture like HEK293, myofibroblasts, cortical neurons, human macrophages, smooth muscle cells, and even on tissue. Fusogenic liposomes enabled highly efficient incorporation of molecules into mammalian cell membranes within 1 to 30 min at fully unchanged cell growth conditions and did not affect cell behavior. We hypothesize that membrane fusions were induced in all cases by the interaction of the positively charged lipids and the delocalized electron system of the aromatic group generating local dipoles and membrane instabilities. Selected applications ranging from basic research to biotechnology are envisaged here.
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000010587 7001_ $$0P:(DE-Juel1)VDB29396$$aHersch, N.$$b1$$uFZJ
000010587 7001_ $$0P:(DE-Juel1)VDB5493$$aDieluweit, S.$$b2$$uFZJ
000010587 7001_ $$0P:(DE-Juel1)130542$$aBiehl, R.$$b3$$uFZJ
000010587 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b4$$uFZJ
000010587 7001_ $$0P:(DE-Juel1)VDB27696$$aHoffmann, B.$$b5$$uFZJ
000010587 773__ $$0PERI:(DE-600)1500067-9$$a10.1021/bc900470y$$gVol. 21, p. 537 - 543$$p537 - 543$$q21<537 - 543$$tBioconjugate chemistry$$v21$$x1043-1802$$y2010
000010587 8567_ $$uhttp://dx.doi.org/10.1021/bc900470y
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000010587 9141_ $$y2010
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