TY  - JOUR
AU  - Csiszar, A.
AU  - Hersch, N.
AU  - Dieluweit, S.
AU  - Biehl, R.
AU  - Merkel, R.
AU  - Hoffmann, B.
TI  - Novel Fusogenic Liposomes for Fluorescent Cell labeling and Membrane Modification
JO  - Bioconjugate chemistry
VL  - 21
SN  - 1043-1802
CY  - Columbus, Ohio
PB  - American Chemical Society
M1  - PreJuSER-10587
SP  - 537 - 543
PY  - 2010
N1  - Record converted from VDB: 12.11.2012
AB  - Efficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications. The fusion efficiency of these liposome systems was tested on single cells in culture like HEK293, myofibroblasts, cortical neurons, human macrophages, smooth muscle cells, and even on tissue. Fusogenic liposomes enabled highly efficient incorporation of molecules into mammalian cell membranes within 1 to 30 min at fully unchanged cell growth conditions and did not affect cell behavior. We hypothesize that membrane fusions were induced in all cases by the interaction of the positively charged lipids and the delocalized electron system of the aromatic group generating local dipoles and membrane instabilities. Selected applications ranging from basic research to biotechnology are envisaged here.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:20184308
UR  - <Go to ISI:>//WOS:000275711600016
DO  - DOI:10.1021/bc900470y
UR  - https://juser.fz-juelich.de/record/10587
ER  -