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@ARTICLE{Csiszar:10587,
author = {Csiszar, A. and Hersch, N. and Dieluweit, S. and Biehl, R.
and Merkel, R. and Hoffmann, B.},
title = {{N}ovel {F}usogenic {L}iposomes for {F}luorescent {C}ell
labeling and {M}embrane {M}odification},
journal = {Bioconjugate chemistry},
volume = {21},
issn = {1043-1802},
address = {Columbus, Ohio},
publisher = {American Chemical Society},
reportid = {PreJuSER-10587},
pages = {537 - 543},
year = {2010},
note = {Record converted from VDB: 12.11.2012},
abstract = {Efficient delivery of biomolecules into membranes of living
cells as well as cell surface modifications are major
biotechnological challenges. Here, novel liposome systems
based on neutral and cationic lipids in combination with
lipids modified by aromatic groups are introduced for such
applications. The fusion efficiency of these liposome
systems was tested on single cells in culture like HEK293,
myofibroblasts, cortical neurons, human macrophages, smooth
muscle cells, and even on tissue. Fusogenic liposomes
enabled highly efficient incorporation of molecules into
mammalian cell membranes within 1 to 30 min at fully
unchanged cell growth conditions and did not affect cell
behavior. We hypothesize that membrane fusions were induced
in all cases by the interaction of the positively charged
lipids and the delocalized electron system of the aromatic
group generating local dipoles and membrane instabilities.
Selected applications ranging from basic research to
biotechnology are envisaged here.},
keywords = {J (WoSType)},
cin = {IBN-4 / IFF-5},
ddc = {540},
cid = {I:(DE-Juel1)VDB802 / I:(DE-Juel1)VDB785},
pnm = {BioSoft: Makromolekulare Systeme und biologische
Informationsverarbeitung / Erneuerbare Energien},
pid = {G:(DE-Juel1)FUEK505 / G:(DE-Juel1)FUEK401},
shelfmark = {Biochemical Research Methods / Biochemistry $\&$ Molecular
Biology / Chemistry, Multidisciplinary / Chemistry, Organic},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:20184308},
UT = {WOS:000275711600016},
doi = {10.1021/bc900470y},
url = {https://juser.fz-juelich.de/record/10587},
}