TY  - JOUR
AU  - Dieluweit, S.
AU  - Csiszar, A.
AU  - Rubner, W.
AU  - Fleischhauer, J.
AU  - Houben, S.
AU  - Merkel, R.
TI  - Mechanical Properties of Bare and Protein-Coated Giant Unilamellar Phospolipid Vesicles. A Comparative Study of Micropipet Aspiration and Atomic Force Microscopy
JO  - Langmuir
VL  - 26
SN  - 0743-7463
CY  - Washington, DC
PB  - ACS Publ.
M1  - PreJuSER-10588
SP  - 11041 - 11049
PY  - 2010
N1  - Record converted from VDB: 12.11.2012
AB  - In this study, protein-coated giant phospholipid vesicles were used to model cell plasma membranes coated by surface protein layers that increase membrane stiffness under mechanical or osmotic stress. These changed mechanical properties like bending stiffness, membrane area compressibility modulus, and effective Young's modulus were determined by micropipet aspiration, while bending stiffness, effective Young's modulus, and effective spring constant of vesicles were analyzed by AFM. The experimental setups, the applied models, and the results using both methods were compared here. As demonstrated before, we found that bare vesicles were best probed by micropipet aspiration due to its high sensitivity. The mechanical properties of vesicles with protein surface layers were, however, better determined by AFM because it enables very local deformations of the membrane with barely any structural damage to the protein layer. Mechanical properties of different species of coating proteins, here streptavidin and avidin, could be clearly distinguished using this technique.
KW  - Mechanics
KW  - Microscopy, Atomic Force
KW  - Models, Theoretical
KW  - Phospholipids: chemistry
KW  - Unilamellar Liposomes: chemistry
KW  - Phospholipids (NLM Chemicals)
KW  - Unilamellar Liposomes (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:20355933
UR  - <Go to ISI:>//WOS:000279239900087
DO  - DOI:10.1021/la1005242
UR  - https://juser.fz-juelich.de/record/10588
ER  -