000010601 001__ 10601
000010601 005__ 20200423202804.0
000010601 0247_ $$2DOI$$a10.1007/s11105-010-0265-2
000010601 0247_ $$2WOS$$aWOS:000296078800010
000010601 037__ $$aPreJuSER-10601
000010601 041__ $$aeng
000010601 082__ $$a570
000010601 084__ $$2WoS$$aBiochemical Research Methods
000010601 084__ $$2WoS$$aPlant Sciences
000010601 1001_ $$0P:(DE-Juel1)VDB86894$$aMuellenborn, C.$$b0$$uFZJ
000010601 245__ $$aAnalysis of Differential Transcript Expression Reveals Time-Dependent Leaf Responses to Sclerotinia seclerotiorum in Wild and Cultivated Sunflower
000010601 260__ $$aDordrecht [u.a.] . Springer$$bSpringer (formerly: Kluwer)$$c2011
000010601 300__ $$a597 - 608
000010601 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000010601 3367_ $$2DataCite$$aOutput Types/Journal article
000010601 3367_ $$00$$2EndNote$$aJournal Article
000010601 3367_ $$2BibTeX$$aARTICLE
000010601 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000010601 3367_ $$2DRIVER$$aarticle
000010601 440_0 $$024603$$aPlant Molecular Biology Reporter$$v29$$y3
000010601 500__ $$aRecord converted from VDB: 12.11.2012
000010601 520__ $$aThe necrotrophic pathogen Sclerotinia sclerotiorum is a causal agent of rot diseases in sunflower and is described as one of the most damaging pathogens of cultivated sunflower. Resistance to this pathogen is found in some genotypes of wild sunflower in particular characterised by significantly reduced lesion lengths in capitulum, stems and leaves. This study was conducted to characterise transcriptomic alterations during interaction of host and pathogen in lesion-surrounding areas of the leaf using differential display RT-PCR and to compare molecular responses between a resistant and a susceptible genotype. Leaves were examined during the first stages of pathogenesis (dpi 2, 3 and 4) after inoculation with S. sclerotiorum. By means of computational analysis of fluorescently labelled expression data, expression patterns were evaluated and significant differentially expressed transcripts were selected. The expression profile revealed that a response measured by the number of significant differentially expressed transcripts differed between the resistant and susceptible genotype in timing. Nine differentially expressed transcripts were successfully sequenced of which two transcripts originated from the mRNA population of the pathogen, two transcripts were derived from the susceptible cultivar of Helianthus annuus and five transcripts were isolated from the resistant genotype of Helianthus maximiliani. Semi-quantitative real-time PCR was accomplished to verify the significant differential expression of the potentially resistance-associated transcripts coumarate-CoA-ligase and cysteine protease transcript in the resistant H. maximiliani accession and differential expression of a chlorophyll-a/b-binding-protein and an S-adenosyl-methionine-synthetase transcript originating from the susceptible H. annuus cultivar.
000010601 536__ $$0G:(DE-Juel1)FUEK407$$2G:(DE-HGF)$$aTerrestrische Umwelt$$cP24$$x0
000010601 588__ $$aDataset connected to Web of Science
000010601 650_7 $$2WoSType$$aJ
000010601 65320 $$2Author$$aWild sunflower
000010601 65320 $$2Author$$aSclerotinia sclerotiorum
000010601 65320 $$2Author$$aResistance
000010601 65320 $$2Author$$aDifferential display
000010601 65320 $$2Author$$aHelianthus maximiliani
000010601 65320 $$2Author$$aHelianthus annuus
000010601 65320 $$2Author$$aCapillary gel electrophoresis
000010601 7001_ $$0P:(DE-Juel1)VDB84847$$aKrause, J.-H.$$b1$$uFZJ
000010601 7001_ $$0P:(DE-Juel1)129301$$aCerboncini, C.$$b2$$uFZJ
000010601 773__ $$0PERI:(DE-600)2018592-3$$a10.1007/s11105-010-0265-2$$gVol. 29, p. 597 - 608$$p597 - 608$$q29<597 - 608$$tPlant molecular biology reporter$$v29$$x0735-9640$$y2011
000010601 8567_ $$uhttp://dx.doi.org/10.1007/s11105-010-0265-2
000010601 8564_ $$uhttps://juser.fz-juelich.de/record/10601/files/FZJ-10601.pdf$$yRestricted$$zPublished final document.
000010601 909CO $$ooai:juser.fz-juelich.de:10601$$pVDB
000010601 9131_ $$0G:(DE-Juel1)FUEK407$$bErde und Umwelt$$kP24$$lTerrestrische Umwelt$$vTerrestrische Umwelt$$x0
000010601 9132_ $$0G:(DE-HGF)POF3-582$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vPlant Science$$x0
000010601 9141_ $$y2011
000010601 915__ $$0StatID:(DE-HGF)0040$$2StatID$$aPeer review unknown
000010601 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000010601 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000010601 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000010601 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000010601 9201_ $$0I:(DE-Juel1)ICG-3-20090406$$d31.10.2010$$gICG$$kICG-3$$lPhytosphäre$$x1
000010601 9201_ $$0I:(DE-Juel1)IBG-2-20101118$$gIBG$$kIBG-2$$lPflanzenwissenschaften$$x2
000010601 970__ $$aVDB:(DE-Juel1)121006
000010601 980__ $$aVDB
000010601 980__ $$aConvertedRecord
000010601 980__ $$ajournal
000010601 980__ $$aI:(DE-Juel1)IBG-2-20101118
000010601 980__ $$aI:(DE-Juel1)IBG-2-20101118
000010601 980__ $$aUNRESTRICTED
000010601 981__ $$aI:(DE-Juel1)IBG-2-20101118
000010601 981__ $$aI:(DE-Juel1)IBG-2-20101118
000010601 981__ $$aI:(DE-Juel1)ICG-3-20090406