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@ARTICLE{Muellenborn:10601,
      author       = {Muellenborn, C. and Krause, J.-H. and Cerboncini, C.},
      title        = {{A}nalysis of {D}ifferential {T}ranscript {E}xpression
                      {R}eveals {T}ime-{D}ependent {L}eaf {R}esponses to
                      {S}clerotinia seclerotiorum in {W}ild and {C}ultivated
                      {S}unflower},
      journal      = {Plant molecular biology reporter},
      volume       = {29},
      issn         = {0735-9640},
      address      = {Dordrecht [u.a.] . Springer},
      publisher    = {Springer (formerly: Kluwer)},
      reportid     = {PreJuSER-10601},
      pages        = {597 - 608},
      year         = {2011},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {The necrotrophic pathogen Sclerotinia sclerotiorum is a
                      causal agent of rot diseases in sunflower and is described
                      as one of the most damaging pathogens of cultivated
                      sunflower. Resistance to this pathogen is found in some
                      genotypes of wild sunflower in particular characterised by
                      significantly reduced lesion lengths in capitulum, stems and
                      leaves. This study was conducted to characterise
                      transcriptomic alterations during interaction of host and
                      pathogen in lesion-surrounding areas of the leaf using
                      differential display RT-PCR and to compare molecular
                      responses between a resistant and a susceptible genotype.
                      Leaves were examined during the first stages of pathogenesis
                      (dpi 2, 3 and 4) after inoculation with S. sclerotiorum. By
                      means of computational analysis of fluorescently labelled
                      expression data, expression patterns were evaluated and
                      significant differentially expressed transcripts were
                      selected. The expression profile revealed that a response
                      measured by the number of significant differentially
                      expressed transcripts differed between the resistant and
                      susceptible genotype in timing. Nine differentially
                      expressed transcripts were successfully sequenced of which
                      two transcripts originated from the mRNA population of the
                      pathogen, two transcripts were derived from the susceptible
                      cultivar of Helianthus annuus and five transcripts were
                      isolated from the resistant genotype of Helianthus
                      maximiliani. Semi-quantitative real-time PCR was
                      accomplished to verify the significant differential
                      expression of the potentially resistance-associated
                      transcripts coumarate-CoA-ligase and cysteine protease
                      transcript in the resistant H. maximiliani accession and
                      differential expression of a chlorophyll-a/b-binding-protein
                      and an S-adenosyl-methionine-synthetase transcript
                      originating from the susceptible H. annuus cultivar.},
      keywords     = {J (WoSType)},
      cin          = {ICG-3 / IBG-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICG-3-20090406 / I:(DE-Juel1)IBG-2-20101118},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Biochemical Research Methods / Plant Sciences},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000296078800010},
      doi          = {10.1007/s11105-010-0265-2},
      url          = {https://juser.fz-juelich.de/record/10601},
}