TY  - JOUR
AU  - Bühler, J.
AU  - Huber, G.
AU  - Schmid, F.
AU  - Blümler, P.
TI  - Analytical model for long-distance tracer-transport in plants
JO  - Journal of theoretical biology
VL  - 270
SN  - 0022-5193
CY  - London
PB  - Academic Press
M1  - PreJuSER-10905
SP  - 70 - 79
PY  - 2011
N1  - We wish to thank Hanno Scharr, Wilfried Wolff, Michael Thorpe and Peter Minchin for helpful discussions. Special thanks go to Siegfried Jahnke for valuable comments and access to the PET data. Jonas Buhler wants to thank Martin Reissel for technical support. Friederike Schmid acknowledges financial support from the MRL of UC Santa Barbara during a sabbatical. This work was partially supported by the MRSEC Program of the National Science Foundation under Award no. DMR05-20415. Finally, Peter Blumler wants to thank Helmut Soltner for an excursion into Laplacian space! Last but not least continuous support from Uli Schurr made this work possible.
AB  - Recent investigations of long-distance transport in plants using non-invasive tracer techniques such as (11)C radiolabeling monitored by positron emission tomography (PET) combined with magnetic resonance imaging (MRI) revealed the need of dedicated methods to allow a quantitative data analysis and comparison of such experiments. A mechanistic compartmental tracer transport model is presented, defined by a linear system of partial differential equations (PDEs). This model simplifies the complexity of axial transport and lateral exchanges in the transport pathways of plants (e.g. the phloem) by simulating transport and reversible exchange within three compartments using just a few parameters which are considered to be constant in space and time. For this system of PDEs an analytical solution in Fourier-space was found allowing a fast and numerically precise evaluation. From the steady-state behavior of the model, the system loss (steadily fixed tracer along the transport conduits) was derived as an additional parameter that can be readily interpreted in a physiological way. The presented framework allows the model to be fitted to spatio-temporal tracer profiles including error and sensitivity analysis of the estimated parameters. This is demonstrated for PET data sets obtained from radish, sugar beet and maize plants.
KW  - Algorithms
KW  - Beta vulgaris: metabolism
KW  - Biological Transport: physiology
KW  - Carbon Radioisotopes: metabolism
KW  - Computer Simulation
KW  - Fourier Analysis
KW  - Magnetic Resonance Imaging
KW  - Models, Biological
KW  - Phloem: metabolism
KW  - Plant Roots: metabolism
KW  - Plant Structures: metabolism
KW  - Plants: metabolism
KW  - Positron-Emission Tomography
KW  - Radioactive Tracers
KW  - Raphanus: metabolism
KW  - Xylem: metabolism
KW  - Zea mays: metabolism
KW  - Carbon Radioisotopes (NLM Chemicals)
KW  - Radioactive Tracers (NLM Chemicals)
KW  - J (WoSType)
KW  - Phloem
KW  - 11C
KW  - Simulation
KW  - Data analysis
KW  - Positron emissiontomography(PET)
LB  - PUB:(DE-HGF)16
C6  - pmid:21056579
UR  - <Go to ISI:>//WOS:000286406700010
DO  - DOI:10.1016/j.jtbi.2010.11.005
UR  - https://juser.fz-juelich.de/record/10905
ER  -