TY  - JOUR
AU  - Lindner, S.N.
AU  - Niederholtmeyer, H.
AU  - Schmitz, K.
AU  - Schobert, S.
AU  - Wendisch, V. F.
TI  - Polyphosphate/ATP-dependent NAD kinase of Corynebacterium glutamicum: biochemical properties and impact of ppnK overexpression on lysine production
JO  - Applied Microbiology and Biotechnology
VL  - 87
SN  - 0175-7598
CY  - Berlin
PB  - Springer
M1  - PreJuSER-10968
SP  - 583 - 593
PY  - 2010
N1  - Record converted from VDB: 12.11.2012
AB  - Nicotinamide adenine dinucleotide phosphate (NADP) is synthesized by phosphorylation of either oxidized or reduced nicotinamide adenine dinucleotide (NAD/NADH). Here, the cg1601/ppnK gene product from Corynebacterium glutamicum genome was purified from recombinant Escherichia coli and enzymatic characterization revealed its activity as a polyphosphate (PolyP)/ATP-dependent NAD kinase (PPNK). PPNK from C. glutamicum was shown to be active as homotetramer accepting PolyP, ATP, and even ADP for phosphorylation of NAD. The catalytic efficiency with ATP as phosphate donor for phosphorylation of NAD was higher than with PolyP. With respect to the chain length of PolyP, PPNK was active with short-chain PolyPs. PPNK activity was independent of bivalent cations when using ATP, but was enhanced by manganese and in particular by magnesium ions. When using PolyP, PPNK required bivalent cations, preferably manganese ions, for activity. PPNK was inhibited by NADP and NADH at concentrations below millimolar. Overexpression of ppnK in C. glutamicum wild type slightly reduced growth and ppnK overexpression in the lysine producing strain DM1729 resulted in a lysine product yield on glucose of 0.136 +/- 0.006 mol lysine (mol glucose)(-1), which was 12% higher than that of the empty vector control strain.
KW  - Adenosine Triphosphate: metabolism
KW  - Amino Acid Sequence
KW  - Bacterial Proteins: chemistry
KW  - Bacterial Proteins: genetics
KW  - Bacterial Proteins: metabolism
KW  - Corynebacterium glutamicum: chemistry
KW  - Corynebacterium glutamicum: enzymology
KW  - Corynebacterium glutamicum: genetics
KW  - Corynebacterium glutamicum: metabolism
KW  - Gene Expression
KW  - Lysine: biosynthesis
KW  - Molecular Sequence Data
KW  - Phosphotransferases: chemistry
KW  - Phosphotransferases: genetics
KW  - Phosphotransferases: metabolism
KW  - Polyphosphates: chemistry
KW  - Polyphosphates: metabolism
KW  - Protein Multimerization
KW  - Sequence Homology, Amino Acid
KW  - Substrate Specificity
KW  - Bacterial Proteins (NLM Chemicals)
KW  - Polyphosphates (NLM Chemicals)
KW  - Adenosine Triphosphate (NLM Chemicals)
KW  - Lysine (NLM Chemicals)
KW  - Phosphotransferases (NLM Chemicals)
KW  - polyphosphate NAD-kinase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:20180116
UR  - <Go to ISI:>//WOS:000277959500020
DO  - DOI:10.1007/s00253-010-2481-y
UR  - https://juser.fz-juelich.de/record/10968
ER  -