Hauptseite > Publikationsdatenbank > Branchpoint expansion in a fully-complementary three-way DNA junction. > print

001     111910
005     20200402205949.0
024 7 _ |2 pmid
|a pmid:22329743
024 7 _ |2 DOI
|a 10.1021/ja211802z
024 7 _ |2 WOS
|a WOS:000302524800037
024 7 _ |a altmetric:631858
|2 altmetric
037 _ _ |a PreJuSER-111910
041 _ _ |a eng
082 _ _ |a 540
084 _ _ |2 WoS
|a Chemistry, Multidisciplinary
100 1 _ |0 P:(DE-HGF)0
|a Sabir, T.
|b 0
245 _ _ |a Branchpoint expansion in a fully-complementary three-way DNA junction.
260 _ _ |a Washington, DC
|b American Chemical Society
|c 2012
300 _ _ |a 6280 - 6285
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |0 25743
|a Journal of the American Chemical Society
|v 134
|y 14
500 _ _ |a We thank the BBSRC for support (BB/G00269X/1); Claus Seidel, Ralf Kuhnemuth, and Stefan Marawske for assistance in developing our MFD setup; Suren Felekyan for help with data analysis; and Jochen Ark for assistance with experiments. S.W.M. acknowledges the award of an EPSRC advanced research fellowship (EP/D073154), and L.M. acknowledges the award of a CSC scholarship.
520 _ _ |a Branched nucleic acid molecules serve as key intermediates in DNA replication, recombination, and repair; architectural elements in RNA; and building blocks and functional components for nanoscience applications. Using a combination of high-resolution single-molecule FRET, time-resolved spectroscopy, and molecular modeling, we have probed the local and global structure of a DNA three-way junction (3WJ) in solution. We found that it adopts a Y-shaped, pyramidal structure, in which the bases adjacent to the branchpoint are unpaired, despite the full Watson-Crick complementarity of the molecule. The unpairing allows a nanoscale cavity to form at the junction center. Our structure accounts for earlier observations made of the structure, flexibility, and reactivity of 3WJs. We anticipate that these results will guide the development of new DNA-based supramolecular receptors and nanosystems.
536 _ _ |0 G:(DE-Juel1)FUEK409
|2 G:(DE-HGF)
|a Funktion und Dysfunktion des Nervensystems
|c P33
|x 0
536 _ _ |0 G:(DE-Juel1)FUEK505
|2 G:(DE-HGF)
|a BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
|c P45
|x 1
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Computer Simulation
650 _ 2 |2 MeSH
|a DNA: chemistry
650 _ 2 |2 MeSH
|a Fluorescence Resonance Energy Transfer: methods
650 _ 2 |2 MeSH
|a Hydrogen Bonding
650 _ 2 |2 MeSH
|a Ions
650 _ 2 |2 MeSH
|a Kinetics
650 _ 2 |2 MeSH
|a Molecular Dynamics Simulation
650 _ 2 |2 MeSH
|a Nanotechnology: methods
650 _ 2 |2 MeSH
|a Nucleic Acid Conformation
650 _ 2 |2 MeSH
|a Nucleic Acids
650 _ 2 |2 MeSH
|a Nucleotides: chemistry
650 _ 2 |2 MeSH
|a RNA: chemistry
650 _ 2 |2 MeSH
|a Reproducibility of Results
650 _ 2 |2 MeSH
|a Spectrophotometry: methods
650 _ 7 |0 0
|2 NLM Chemicals
|a Ions
650 _ 7 |0 0
|2 NLM Chemicals
|a Nucleic Acids
650 _ 7 |0 0
|2 NLM Chemicals
|a Nucleotides
650 _ 7 |0 63231-63-0
|2 NLM Chemicals
|a RNA
650 _ 7 |0 9007-49-2
|2 NLM Chemicals
|a DNA
650 _ 7 |2 WoSType
|a J
700 1 _ |0 P:(DE-HGF)0
|a Toulmin, A.
|b 1
700 1 _ |0 P:(DE-HGF)0
|a Ma, L.
|b 2
700 1 _ |0 P:(DE-HGF)0
|a Jones, A.C.
|b 3
700 1 _ |0 P:(DE-HGF)0
|a McGlynn, P.
|b 4
700 1 _ |0 P:(DE-Juel1)132018
|a Schröder, G.F.
|b 5
|u FZJ
700 1 _ |0 P:(DE-HGF)0
|a Magennis, S.W.
|b 6
773 _ _ |0 PERI:(DE-600)1472210-0
|a 10.1021/ja211802z
|g Vol. 134, p. 6280 - 6285
|p 6280 - 6285
|q 134<6280 - 6285
|t Journal of the American Chemical Society
|v 134
|x 0002-7863
|y 2012
856 7 _ |u http://dx.doi.org/10.1021/ja211802z
909 C O |o oai:juser.fz-juelich.de:111910
|p VDB
913 1 _ |0 G:(DE-Juel1)FUEK409
|1 G:(DE-HGF)POF2-330
|2 G:(DE-HGF)POF2-300
|a DE-HGF
|b Gesundheit
|k P33
|l Funktion und Dysfunktion des Nervensystems
|v Funktion und Dysfunktion des Nervensystems
|x 0
913 1 _ |0 G:(DE-Juel1)FUEK505
|1 G:(DE-HGF)POF2-450
|2 G:(DE-HGF)POF2-400
|a DE-HGF
|b Schlüsseltechnologien
|k P45
|l Biologische Informationsverarbeitung
|v BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
|x 1
913 2 _ |0 G:(DE-HGF)POF3-551
|1 G:(DE-HGF)POF3-550
|2 G:(DE-HGF)POF3-500
|a DE-HGF
|b Key Technologies
|l BioSoft Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences
|v Functional Macromolecules and Complexes
|x 0
914 1 _ |y 2012
915 _ _ |0 StatID:(DE-HGF)0010
|2 StatID
|a JCR/ISI refereed
915 _ _ |0 StatID:(DE-HGF)0100
|2 StatID
|a JCR
915 _ _ |0 StatID:(DE-HGF)0110
|2 StatID
|a WoS
|b Science Citation Index
915 _ _ |0 StatID:(DE-HGF)0111
|2 StatID
|a WoS
|b Science Citation Index Expanded
915 _ _ |0 StatID:(DE-HGF)0150
|2 StatID
|a DBCoverage
|b Web of Science Core Collection
915 _ _ |0 StatID:(DE-HGF)0199
|2 StatID
|a DBCoverage
|b Thomson Reuters Master Journal List
915 _ _ |0 StatID:(DE-HGF)0200
|2 StatID
|a DBCoverage
|b SCOPUS
915 _ _ |0 StatID:(DE-HGF)0300
|2 StatID
|a DBCoverage
|b Medline
915 _ _ |0 StatID:(DE-HGF)0310
|2 StatID
|a DBCoverage
|b NCBI Molecular Biology Database
915 _ _ |0 StatID:(DE-HGF)0420
|2 StatID
|a Nationallizenz
915 _ _ |0 StatID:(DE-HGF)1030
|2 StatID
|a DBCoverage
|b Current Contents - Life Sciences
915 _ _ |0 StatID:(DE-HGF)1050
|2 StatID
|a DBCoverage
|b BIOSIS Previews
915 _ _ |0 StatID:(DE-HGF)1150
|2 StatID
|a DBCoverage
|b Current Contents - Physical, Chemical and Earth Sciences
920 1 _ |0 I:(DE-Juel1)ICS-6-20110106
|g ICS
|k ICS-6
|l Strukturbiochemie
|x 0
970 _ _ |a VDB:(DE-Juel1)140541
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)ICS-6-20110106
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBI-7-20200312

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21