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000111979 0247_ $$2DOI$$a10.1371/journal.pone.0035458
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000111979 1001_ $$0P:(DE-HGF)0$$aKubicek, J.$$b0
000111979 245__ $$aControlled In Meso Phase Crystallization -- A Method for the Structural Investigation of Membrane Proteins
000111979 260__ $$aLawrence, Kan.$$bPLoS$$c2012
000111979 300__ $$ae35458
000111979 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000111979 520__ $$aWe investigated in meso crystallization of membrane proteins to develop a fast screening technology which combines features of the well established classical vapor diffusion experiment with the batch meso phase crystallization, but without premixing of protein and monoolein. It inherits the advantages of both methods, namely (i) the stabilization of membrane proteins in the meso phase, (ii) the control of hydration level and additive concentration by vapor diffusion. The new technology (iii) significantly simplifies in meso crystallization experiments and allows the use of standard liquid handling robots suitable for 96 well formats. CIMP crystallization furthermore allows (iv) direct monitoring of phase transformation and crystallization events. Bacteriorhodopsin (BR) crystals of high quality and diffraction up to 1.3 Å resolution have been obtained in this approach. CIMP and the developed consumables and protocols have been successfully applied to obtain crystals of sensory rhodopsin II (SRII) from Halobacterium salinarum for the first time.
000111979 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
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000111979 650_2 $$2MeSH$$aBacteriorhodopsins: chemistry
000111979 650_2 $$2MeSH$$aCrystallization
000111979 650_2 $$2MeSH$$aCrystallography, X-Ray: methods
000111979 650_2 $$2MeSH$$aGlycerides: chemistry
000111979 650_2 $$2MeSH$$aHalobacterium salinarum
000111979 650_2 $$2MeSH$$aHalorhodopsins: chemistry
000111979 650_2 $$2MeSH$$aMembrane Proteins: chemistry
000111979 650_2 $$2MeSH$$aPhase Transition
000111979 650_2 $$2MeSH$$aProtein Stability
000111979 650_2 $$2MeSH$$aSensory Rhodopsins: chemistry
000111979 650_7 $$00$$2NLM Chemicals$$aGlycerides
000111979 650_7 $$00$$2NLM Chemicals$$aHalorhodopsins
000111979 650_7 $$00$$2NLM Chemicals$$aMembrane Proteins
000111979 650_7 $$00$$2NLM Chemicals$$aSensory Rhodopsins
000111979 650_7 $$00$$2NLM Chemicals$$asensory rhodopsin II protein, archaeal
000111979 650_7 $$025496-72-4$$2NLM Chemicals$$amonoolein
000111979 650_7 $$053026-44-1$$2NLM Chemicals$$aBacteriorhodopsins
000111979 7001_ $$0P:(DE-HGF)0$$aSchlesinger, R.$$b1
000111979 7001_ $$0P:(DE-Juel1)VDB59848$$aBaeken, C.$$b2$$uFZJ
000111979 7001_ $$0P:(DE-Juel1)131957$$aBüldt, G.$$b3$$uFZJ
000111979 7001_ $$0P:(DE-HGF)0$$aSchäfer, F.$$b4
000111979 7001_ $$0P:(DE-Juel1)VDB886$$aLabahn, J.$$b5$$uFZJ
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000111979 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334905
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