TY  - JOUR
AU  - Kubicek, J.
AU  - Schlesinger, R.
AU  - Baeken, C.
AU  - Büldt, G.
AU  - Schäfer, F.
AU  - Labahn, J.
TI  - Controlled In Meso Phase Crystallization -- A Method for the Structural Investigation of Membrane Proteins
JO  - PLoS one
VL  - 7
SN  - 1932-6203
CY  - Lawrence, Kan.
PB  - PLoS
M1  - PreJuSER-111979
SP  - e35458
PY  - 2012
N1  - Record converted from VDB: 16.11.2012
AB  - We investigated in meso crystallization of membrane proteins to develop a fast screening technology which combines features of the well established classical vapor diffusion experiment with the batch meso phase crystallization, but without premixing of protein and monoolein. It inherits the advantages of both methods, namely (i) the stabilization of membrane proteins in the meso phase, (ii) the control of hydration level and additive concentration by vapor diffusion. The new technology (iii) significantly simplifies in meso crystallization experiments and allows the use of standard liquid handling robots suitable for 96 well formats. CIMP crystallization furthermore allows (iv) direct monitoring of phase transformation and crystallization events. Bacteriorhodopsin (BR) crystals of high quality and diffraction up to 1.3 Å resolution have been obtained in this approach. CIMP and the developed consumables and protocols have been successfully applied to obtain crystals of sensory rhodopsin II (SRII) from Halobacterium salinarum for the first time.
KW  - Bacteriorhodopsins: chemistry
KW  - Crystallization
KW  - Crystallography, X-Ray: methods
KW  - Glycerides: chemistry
KW  - Halobacterium salinarum
KW  - Halorhodopsins: chemistry
KW  - Membrane Proteins: chemistry
KW  - Phase Transition
KW  - Protein Stability
KW  - Sensory Rhodopsins: chemistry
KW  - Glycerides (NLM Chemicals)
KW  - Halorhodopsins (NLM Chemicals)
KW  - Membrane Proteins (NLM Chemicals)
KW  - Sensory Rhodopsins (NLM Chemicals)
KW  - sensory rhodopsin II protein, archaeal (NLM Chemicals)
KW  - monoolein (NLM Chemicals)
KW  - Bacteriorhodopsins (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:22536388
C2  - pmc:PMC3334905
UR  - <Go to ISI:>//WOS:000305336200043
DO  - DOI:10.1371/journal.pone.0035458
UR  - https://juser.fz-juelich.de/record/111979
ER  -