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000011656 0247_ $$2pmid$$apmid:20204663
000011656 0247_ $$2DOI$$a10.1007/s00449-010-0410-1
000011656 0247_ $$2WOS$$aWOS:000280892700010
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000011656 041__ $$aeng
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000011656 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000011656 084__ $$2WoS$$aEngineering, Chemical
000011656 1001_ $$0P:(DE-HGF)0$$aBartek, T.$$b0
000011656 245__ $$aStudies on substrate utilisation in L-valine-producing Corynebacterium glutamicum strains deficient in pyruvate dehydrogenase complex
000011656 260__ $$aBerlin$$bSpringer$$c2010
000011656 300__ $$a873 - 883
000011656 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000011656 440_0 $$07282$$aBioprocess and Biosystems Engineering$$v33$$x1615-7591$$y7
000011656 500__ $$aThis work was financially supported by the Fachagentur Nachwachsende Rohstoffe of the BMVEL-Federal Ministry of Food, Agriculture and Consumer Protection-(grant 04NR003/22000304) and by Evonik Degussa GmbH. The authors wish to thank Verena Engels from IBT 1 of Forschungszentrum Julich GmbH as well as Robert Gerstmeir and Andreas Karau from Evonik Degussa GmbH for fruitful cooperation and the valuable discussion of results, and Pia Makus for her assistance in performing the experiments.
000011656 520__ $$aThe pyruvate dehydrogenase complex was deleted to increase precursor availability in Corynebacterium glutamicum strains overproducing L: -valine. The resulting auxotrophy is treated by adding acetate in addition glucose for growth, resulting in the puzzling fact of gluconeogenic growth with strongly reduced glucose uptake in the presence of acetate in the medium. This result was proven by intracellular metabolite analysis and labelling experiments. To increase productivity, the SugR protein involved in negative regulation of the phosphotransferase system, was inactivated, resulting in enhanced consumption of glucose. However, the surplus in substrate uptake was not converted to L-valine; instead, the formation of up to 289 microM xylulose was observed for the first time in C. glutamicum. As an alternative to the genetic engineering solution, a straightforward process engineering approach is proposed. Acetate limitation resulted in a more efficient use of acetate as cosubstrate, shown by an increased biomass yield Y(X/Ac) and improved L-valine formation.
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000011656 65320 $$2Author$$aCorynebacterium glutamicum
000011656 65320 $$2Author$$aL-Valine
000011656 65320 $$2Author$$aPyruvate dehydrogenase complex
000011656 65320 $$2Author$$aSubstrate uptake
000011656 65320 $$2Author$$aFermentation process development
000011656 65320 $$2Author$$aXylulose
000011656 650_2 $$2MeSH$$aCorynebacterium: classification
000011656 650_2 $$2MeSH$$aCorynebacterium: metabolism
000011656 650_2 $$2MeSH$$aGenetic Enhancement: methods
000011656 650_2 $$2MeSH$$aPyruvate Dehydrogenase Complex: genetics
000011656 650_2 $$2MeSH$$aPyruvate Dehydrogenase Complex: metabolism
000011656 650_2 $$2MeSH$$aSpecies Specificity
000011656 650_2 $$2MeSH$$aSubstrate Specificity
000011656 650_2 $$2MeSH$$aValine: biosynthesis
000011656 650_7 $$00$$2NLM Chemicals$$aPyruvate Dehydrogenase Complex
000011656 650_7 $$07004-03-7$$2NLM Chemicals$$aValine
000011656 650_7 $$2WoSType$$aJ
000011656 7001_ $$0P:(DE-HGF)0$$aRudolf, C.$$b1
000011656 7001_ $$0P:(DE-HGF)0$$aKerßen, U.$$b2
000011656 7001_ $$0P:(DE-Juel1)VDB61576$$aKlein, B.$$b3$$uFZJ
000011656 7001_ $$0P:(DE-HGF)0$$aBlombach, B.$$b4
000011656 7001_ $$0P:(DE-HGF)0$$aLang, S.$$b5
000011656 7001_ $$0P:(DE-HGF)0$$aEikmanns, B.$$b6
000011656 7001_ $$0P:(DE-Juel1)129053$$aOldiges, M.$$b7$$uFZJ
000011656 773__ $$0PERI:(DE-600)1476357-6$$a10.1007/s00449-010-0410-1$$gVol. 33, p. 873 - 883$$p873 - 883$$q33<873 - 883$$tBioprocess and biosystems engineering$$v33$$x1615-7591$$y2010
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000011656 9141_ $$y2010
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000011656 9132_ $$0G:(DE-HGF)POF3-581$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vBiotechnology$$x0
000011656 9201_ $$0I:(DE-Juel1)VDB56$$gIBT$$kIBT-2$$lBiotechnologie 2$$x0$$zab 31.10.10 weitergeführt IBG-1
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