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@ARTICLE{Bartek:11656,
      author       = {Bartek, T. and Rudolf, C. and Kerßen, U. and Klein, B. and
                      Blombach, B. and Lang, S. and Eikmanns, B. and Oldiges, M.},
      title        = {{S}tudies on substrate utilisation in {L}-valine-producing
                      {C}orynebacterium glutamicum strains deficient in pyruvate
                      dehydrogenase complex},
      journal      = {Bioprocess and biosystems engineering},
      volume       = {33},
      issn         = {1615-7591},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {PreJuSER-11656},
      pages        = {873 - 883},
      year         = {2010},
      note         = {This work was financially supported by the Fachagentur
                      Nachwachsende Rohstoffe of the BMVEL-Federal Ministry of
                      Food, Agriculture and Consumer Protection-(grant
                      04NR003/22000304) and by Evonik Degussa GmbH. The authors
                      wish to thank Verena Engels from IBT 1 of Forschungszentrum
                      Julich GmbH as well as Robert Gerstmeir and Andreas Karau
                      from Evonik Degussa GmbH for fruitful cooperation and the
                      valuable discussion of results, and Pia Makus for her
                      assistance in performing the experiments.},
      abstract     = {The pyruvate dehydrogenase complex was deleted to increase
                      precursor availability in Corynebacterium glutamicum strains
                      overproducing L: -valine. The resulting auxotrophy is
                      treated by adding acetate in addition glucose for growth,
                      resulting in the puzzling fact of gluconeogenic growth with
                      strongly reduced glucose uptake in the presence of acetate
                      in the medium. This result was proven by intracellular
                      metabolite analysis and labelling experiments. To increase
                      productivity, the SugR protein involved in negative
                      regulation of the phosphotransferase system, was
                      inactivated, resulting in enhanced consumption of glucose.
                      However, the surplus in substrate uptake was not converted
                      to L-valine; instead, the formation of up to 289 microM
                      xylulose was observed for the first time in C. glutamicum.
                      As an alternative to the genetic engineering solution, a
                      straightforward process engineering approach is proposed.
                      Acetate limitation resulted in a more efficient use of
                      acetate as cosubstrate, shown by an increased biomass yield
                      Y(X/Ac) and improved L-valine formation.},
      keywords     = {Corynebacterium: classification / Corynebacterium:
                      metabolism / Genetic Enhancement: methods / Pyruvate
                      Dehydrogenase Complex: genetics / Pyruvate Dehydrogenase
                      Complex: metabolism / Species Specificity / Substrate
                      Specificity / Valine: biosynthesis / Pyruvate Dehydrogenase
                      Complex (NLM Chemicals) / Valine (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IBT-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK410},
      shelfmark    = {Biotechnology $\&$ Applied Microbiology / Engineering,
                      Chemical},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:20204663},
      UT           = {WOS:000280892700010},
      doi          = {10.1007/s00449-010-0410-1},
      url          = {https://juser.fz-juelich.de/record/11656},
}