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000011819 0247_ $$2DOI$$a10.1016/j.ab.2010.08.028
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000011819 084__ $$2WoS$$aBiochemical Research Methods
000011819 084__ $$2WoS$$aBiochemistry & Molecular Biology
000011819 084__ $$2WoS$$aChemistry, Analytical
000011819 1001_ $$0P:(DE-Juel1)VDB89237$$aGlück, J.M.$$b0$$uFZJ
000011819 245__ $$aNanodiscs allow the use of integral membrane proteins as analytes in surface plasmon resonance studies
000011819 260__ $$aSan Diego, Calif.$$bElsevier$$c2011
000011819 300__ $$a46 - 52
000011819 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000011819 440_0 $$0362$$aAnalytical Biochemistry$$v408$$x0003-2697$$y1
000011819 500__ $$aRecord converted from VDB: 12.11.2012
000011819 520__ $$aNanodiscs are small-sized and flat model membranes that provide a close to native environment for reconstitution of integral membrane proteins. Incorporation of membrane proteins into nanodiscs results in water-soluble proteolipid particles making the membrane proteins amenable to a multitude of bioanalytical techniques originally developed for soluble proteins. The transmembrane domain of the human CD4 receptor was fused to ubiquitin with a preceding N-terminal decahistidine tag. The resulting integral membrane protein was incorporated into nanodiscs. Binding of the nanodisc-inserted histidine-tagged protein to a monoclonal anti-pentahistidine antibody was quantified using surface plasmon resonance (SPR) experiments. For the first time, a membrane-inserted transmembrane protein was employed as analyte while the antibody served as ligand immobilized on the sensor chip surface. SPR experiments were conducted in single-cycle mode. We demonstrate that the nanodisc-incorporated membrane protein showed nearly identical affinity toward the antibody as did the soluble decahistidine-tagged ubiquitin studied in a comparative experiment. Advantages of the new experimental setup and potential applications are discussed.
000011819 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000011819 536__ $$0G:(DE-Juel1)FUEK505$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x1
000011819 588__ $$aDataset connected to Web of Science, Pubmed
000011819 650_2 $$2MeSH$$aAntibodies, Monoclonal: immunology
000011819 650_2 $$2MeSH$$aAntigens, CD4: chemistry
000011819 650_2 $$2MeSH$$aAntigens, CD4: genetics
000011819 650_2 $$2MeSH$$aAntigens, CD4: metabolism
000011819 650_2 $$2MeSH$$aBiosensing Techniques: methods
000011819 650_2 $$2MeSH$$aHumans
000011819 650_2 $$2MeSH$$aKinetics
000011819 650_2 $$2MeSH$$aMembrane Proteins: chemistry
000011819 650_2 $$2MeSH$$aMembrane Proteins: genetics
000011819 650_2 $$2MeSH$$aMembrane Proteins: metabolism
000011819 650_2 $$2MeSH$$aNanostructures: chemistry
000011819 650_2 $$2MeSH$$aOligopeptides: chemistry
000011819 650_2 $$2MeSH$$aOligopeptides: genetics
000011819 650_2 $$2MeSH$$aOligopeptides: metabolism
000011819 650_2 $$2MeSH$$aProtein Binding
000011819 650_2 $$2MeSH$$aRecombinant Fusion Proteins: chemistry
000011819 650_2 $$2MeSH$$aRecombinant Fusion Proteins: genetics
000011819 650_2 $$2MeSH$$aRecombinant Fusion Proteins: metabolism
000011819 650_2 $$2MeSH$$aSurface Plasmon Resonance: methods
000011819 650_2 $$2MeSH$$aUbiquitin: chemistry
000011819 650_2 $$2MeSH$$aUbiquitin: genetics
000011819 650_2 $$2MeSH$$aUbiquitin: metabolism
000011819 650_7 $$00$$2NLM Chemicals$$aAntibodies, Monoclonal
000011819 650_7 $$00$$2NLM Chemicals$$aAntigens, CD4
000011819 650_7 $$00$$2NLM Chemicals$$aMembrane Proteins
000011819 650_7 $$00$$2NLM Chemicals$$aOligopeptides
000011819 650_7 $$00$$2NLM Chemicals$$aRecombinant Fusion Proteins
000011819 650_7 $$00$$2NLM Chemicals$$aUbiquitin
000011819 650_7 $$2WoSType$$aJ
000011819 65320 $$2Author$$aSurface plasmon resonance
000011819 65320 $$2Author$$aBiacore
000011819 65320 $$2Author$$aSingle-cycle kinetics
000011819 65320 $$2Author$$aNanodisc
000011819 7001_ $$0P:(DE-Juel1)132009$$aKoenig, B. W.$$b1$$uFZJ
000011819 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b2$$uFZJ
000011819 773__ $$0PERI:(DE-600)1461105-3$$a10.1016/j.ab.2010.08.028$$gVol. 408, p. 46 - 52$$p46 - 52$$q408<46 - 52$$tAnalytical biochemistry$$v408$$x0003-2697$$y2011
000011819 8567_ $$uhttp://dx.doi.org/10.1016/j.ab.2010.08.028
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000011819 9131_ $$0G:(DE-Juel1)FUEK409$$bGesundheit$$kP33$$lFunktion und Dysfunktion des Nervensystems$$vFunktion und Dysfunktion des Nervensystems$$x0
000011819 9131_ $$0G:(DE-Juel1)FUEK505$$bSchlüsseltechnologien$$kP45$$lBiologische Informationsverarbeitung$$vBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$x1
000011819 9132_ $$0G:(DE-HGF)POF3-551$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lBioSoft  Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vFunctional Macromolecules and Complexes$$x0
000011819 9141_ $$y2011
000011819 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000011819 9201_ $$0I:(DE-Juel1)ICS-6-20110106$$gICS$$kICS-6$$lStrukturbiochemie$$x0
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