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000011921 0247_ $$2DOI$$a10.1039/c0ja00055h
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000011921 041__ $$aeng
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000011921 084__ $$2WoS$$aChemistry, Analytical
000011921 084__ $$2WoS$$aSpectroscopy
000011921 1001_ $$0P:(DE-HGF)0$$aPozebon, D.$$b0
000011921 245__ $$aBioimaging of metals in thin mouse brain section by laser ablation inductively coupled plamsa mass spectrometry: novel online quantification strategy using aqueous standards
000011921 260__ $$aCambridge$$bChemSoc$$c2010
000011921 300__ $$a1739 - 1744
000011921 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000011921 440_0 $$03023$$aJournal of Analytical Atomic Spectrometry$$v25$$x0267-9477$$y11
000011921 500__ $$aDirce Pozebon would like to thank CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) for financial support. The authors thank Jurgen Srega and Meike Hamester (Thermo Fisher Scientific) for instrumental support of new BrainMet (BrainMet-Bioimaging of Metals and Metallomics) laboratory at Research Centre Juelich (www.brainmet.com).
000011921 520__ $$aA novel solution-based calibration method for quantitative spatial resolved distribution analysis (imaging) of elements in thin biological tissue sections by LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry) is described. A dual flow of the carrier and nebulizer gas is used to transport the aerosol of the laser ablated solid sample (brain tissue) and that of the nebulized aqueous standard into inductively coupled plasma (ICP) source, respectively. Both aerosols are introduced separately in the injector tube inside a special ICP torch and then mixed in the inductively coupled plasma. Calibration curves were obtained via two different calibration strategies: (i) solution based calibration and (ii) with a set of well characterized homogeneous brain laboratory standards. In the first approach matrix matching is performed by solution nebulization of a series of aqueous standards with defined analyte concentrations and simultaneous laser ablation of brain homogenate followed by nebulization of 2% (v/v) HNO3 and laser ablation of a whole brain slice (line by line). In the second approach of calibration a set of brain homogenates with defined analyte concentrations is analyzed by LA-ICP-MS followed by the imaging of brain tissue under the same experimental conditions (dry plasma). Calibration curves of elements of interest (e. g., Li, Na, Al, K, Ca, Ti, V, Mn, Ni, Co, Cr, Cu, Zn, As, Se, Rb, Sr, Y, Cd, Ba, La, Ce, Nd, Gd, Hg, Pb, Bi and U) were obtained using (i) aqueous standards or (ii) the set of synthetic laboratory standards prepared from a mouse brain homogenate doped with elements at defined concentrations. The ratio of the slope of the calibration curves (obtained by using aqueous standards and solid standards) was applied to correct the differences of sensitivity among ICP-MS and LA-ICP-MS. Quantitative images of Li, Mn, Fe, Cu, Zn and Rb in mouse brain were obtained under wet plasma condition (nebulization of HNO3 solution in parallel with ablation of solid brain sample).
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000011921 7001_ $$0P:(DE-HGF)0$$aDressler, V.L.$$b1
000011921 7001_ $$0P:(DE-HGF)0$$aMesko, M.F.$$b2
000011921 7001_ $$0P:(DE-Juel1)138474$$aMatusch, A.$$b3$$uFZJ
000011921 7001_ $$0P:(DE-Juel1)VDB2662$$aBecker, J. S.$$b4$$uFZJ
000011921 773__ $$0PERI:(DE-600)1484654-8$$a10.1039/c0ja00055h$$gVol. 25, p. 1739 - 1744$$p1739 - 1744$$q25<1739 - 1744$$tJournal of analytical atomic spectrometry$$v25$$x0267-9477$$y2010
000011921 8567_ $$uhttp://dx.doi.org/10.1039/c0ja00055h
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000011921 9141_ $$y2010
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