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@ARTICLE{Pozebon:11921,
      author       = {Pozebon, D. and Dressler, V.L. and Mesko, M.F. and Matusch,
                      A. and Becker, J. S.},
      title        = {{B}ioimaging of metals in thin mouse brain section by laser
                      ablation inductively coupled plamsa mass spectrometry: novel
                      online quantification strategy using aqueous standards},
      journal      = {Journal of analytical atomic spectrometry},
      volume       = {25},
      issn         = {0267-9477},
      address      = {Cambridge},
      publisher    = {ChemSoc},
      reportid     = {PreJuSER-11921},
      pages        = {1739 - 1744},
      year         = {2010},
      note         = {Dirce Pozebon would like to thank CAPES (Coordenacao de
                      Aperfeicoamento de Pessoal de Nivel Superior) for financial
                      support. The authors thank Jurgen Srega and Meike Hamester
                      (Thermo Fisher Scientific) for instrumental support of new
                      BrainMet (BrainMet-Bioimaging of Metals and Metallomics)
                      laboratory at Research Centre Juelich (www.brainmet.com).},
      abstract     = {A novel solution-based calibration method for quantitative
                      spatial resolved distribution analysis (imaging) of elements
                      in thin biological tissue sections by LA-ICP-MS (laser
                      ablation inductively coupled plasma mass spectrometry) is
                      described. A dual flow of the carrier and nebulizer gas is
                      used to transport the aerosol of the laser ablated solid
                      sample (brain tissue) and that of the nebulized aqueous
                      standard into inductively coupled plasma (ICP) source,
                      respectively. Both aerosols are introduced separately in the
                      injector tube inside a special ICP torch and then mixed in
                      the inductively coupled plasma. Calibration curves were
                      obtained via two different calibration strategies: (i)
                      solution based calibration and (ii) with a set of well
                      characterized homogeneous brain laboratory standards. In the
                      first approach matrix matching is performed by solution
                      nebulization of a series of aqueous standards with defined
                      analyte concentrations and simultaneous laser ablation of
                      brain homogenate followed by nebulization of $2\%$ (v/v)
                      HNO3 and laser ablation of a whole brain slice (line by
                      line). In the second approach of calibration a set of brain
                      homogenates with defined analyte concentrations is analyzed
                      by LA-ICP-MS followed by the imaging of brain tissue under
                      the same experimental conditions (dry plasma). Calibration
                      curves of elements of interest (e. g., Li, Na, Al, K, Ca,
                      Ti, V, Mn, Ni, Co, Cr, Cu, Zn, As, Se, Rb, Sr, Y, Cd, Ba,
                      La, Ce, Nd, Gd, Hg, Pb, Bi and U) were obtained using (i)
                      aqueous standards or (ii) the set of synthetic laboratory
                      standards prepared from a mouse brain homogenate doped with
                      elements at defined concentrations. The ratio of the slope
                      of the calibration curves (obtained by using aqueous
                      standards and solid standards) was applied to correct the
                      differences of sensitivity among ICP-MS and LA-ICP-MS.
                      Quantitative images of Li, Mn, Fe, Cu, Zn and Rb in mouse
                      brain were obtained under wet plasma condition (nebulization
                      of HNO3 solution in parallel with ablation of solid brain
                      sample).},
      keywords     = {J (WoSType)},
      cin          = {INM-2 / ZCH},
      ddc          = {540},
      cid          = {I:(DE-Juel1)INM-2-20090406 / I:(DE-Juel1)ZCH-20090406},
      pnm          = {Funktion und Dysfunktion des Nervensystems},
      pid          = {G:(DE-Juel1)FUEK409},
      shelfmark    = {Chemistry, Analytical / Spectroscopy},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000283150100011},
      doi          = {10.1039/c0ja00055h},
      url          = {https://juser.fz-juelich.de/record/11921},
}