TY  - JOUR
AU  - Becker, J. S.
AU  - Breuer, U.
AU  - Hsieh, H.-F.
AU  - Osterholt, T.
AU  - Kumtabtim, U.
AU  - Wu, B.
AU  - Matusch, A.
AU  - Caruso, J.A.
AU  - Qin, Z.
TI  - Bioimaging of Metals and Biomolecules in Mouse Heart by Laser Ablation Inductively Coupled Plasma Mass Spectrometry and Secondary Ion Mass Spectrometry
JO  - Analytical chemistry
VL  - 82
SN  - 0003-2700
CY  - Columbus, Ohio
PB  - American Chemical Society
M1  - PreJuSER-11987
SP  - 9528 - 9533
PY  - 2010
N1  - Record converted from VDB: 12.11.2012
AB  - Bioimaging mass spectrometric techniques allow direct mapping of metal and biomolecule distributions with high spatial resolution in biological tissue. In this study laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) was used for imaging of transition metals (Fe, Cu, Zn, Mn, and Ti), alkali and alkaline-earth metals (Na, K, Mg, and Ca, respectively), and selected nonmetals (such as C, P, and S) in native cryosections of mouse heart. The metal and nonmetal images clearly illustrated the shape and the anatomy of the samples. Zinc and copper were inhomogeneously distributed with average concentrations of 26 and 11 μg g(-1), respectively. Titanium and manganese were detected at concentrations reaching 1 and 2 μg g(-1), respectively. The highest regional metal concentration of 360 μg g(-1)was observed for iron in blood present in the lumen of the aorta. Secondary ion mass spectrometry (SIMS) as an elemental and biomolecular mass spectrometric technique was employed for imaging of Na, K, and selected biomolecules (e.g., phosphocholine, choline, cholesterol) in adjacent sections. Here, two different bioimaging techniques, LA-ICPMS and SIMS, were combined for the first time, yielding novel information on both elemental and biomolecular distributions.
KW  - Animals
KW  - Bismuth: chemistry
KW  - Feasibility Studies
KW  - Lasers
KW  - Male
KW  - Mass Spectrometry: methods
KW  - Metals: metabolism
KW  - Mice
KW  - Molecular Imaging: methods
KW  - Myocardium: metabolism
KW  - Metals (NLM Chemicals)
KW  - Bismuth (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:20977196
UR  - <Go to ISI:>//WOS:000284080500056
DO  - DOI:10.1021/ac102256q
UR  - https://juser.fz-juelich.de/record/11987
ER  -