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@ARTICLE{Becker:11987,
author = {Becker, J. S. and Breuer, U. and Hsieh, H.-F. and
Osterholt, T. and Kumtabtim, U. and Wu, B. and Matusch, A.
and Caruso, J.A. and Qin, Z.},
title = {{B}ioimaging of {M}etals and {B}iomolecules in {M}ouse
{H}eart by {L}aser {A}blation {I}nductively {C}oupled
{P}lasma {M}ass {S}pectrometry and {S}econdary {I}on {M}ass
{S}pectrometry},
journal = {Analytical chemistry},
volume = {82},
issn = {0003-2700},
address = {Columbus, Ohio},
publisher = {American Chemical Society},
reportid = {PreJuSER-11987},
pages = {9528 - 9533},
year = {2010},
note = {Record converted from VDB: 12.11.2012},
abstract = {Bioimaging mass spectrometric techniques allow direct
mapping of metal and biomolecule distributions with high
spatial resolution in biological tissue. In this study laser
ablation inductively coupled plasma mass spectrometry
(LA-ICPMS) was used for imaging of transition metals (Fe,
Cu, Zn, Mn, and Ti), alkali and alkaline-earth metals (Na,
K, Mg, and Ca, respectively), and selected nonmetals (such
as C, P, and S) in native cryosections of mouse heart. The
metal and nonmetal images clearly illustrated the shape and
the anatomy of the samples. Zinc and copper were
inhomogeneously distributed with average concentrations of
26 and 11 μg g(-1), respectively. Titanium and manganese
were detected at concentrations reaching 1 and 2 μg g(-1),
respectively. The highest regional metal concentration of
360 μg g(-1)was observed for iron in blood present in the
lumen of the aorta. Secondary ion mass spectrometry (SIMS)
as an elemental and biomolecular mass spectrometric
technique was employed for imaging of Na, K, and selected
biomolecules (e.g., phosphocholine, choline, cholesterol) in
adjacent sections. Here, two different bioimaging
techniques, LA-ICPMS and SIMS, were combined for the first
time, yielding novel information on both elemental and
biomolecular distributions.},
keywords = {Animals / Bismuth: chemistry / Feasibility Studies / Lasers
/ Male / Mass Spectrometry: methods / Metals: metabolism /
Mice / Molecular Imaging: methods / Myocardium: metabolism /
Metals (NLM Chemicals) / Bismuth (NLM Chemicals) / J
(WoSType)},
cin = {ZCH / INM-1 / INM-2},
ddc = {540},
cid = {I:(DE-Juel1)ZCH-20090406 / I:(DE-Juel1)INM-1-20090406 /
I:(DE-Juel1)INM-2-20090406},
pnm = {Funktion und Dysfunktion des Nervensystems (FUEK409) /
89571 - Connectivity and Activity (POF2-89571)},
pid = {G:(DE-Juel1)FUEK409 / G:(DE-HGF)POF2-89571},
shelfmark = {Chemistry, Analytical},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:20977196},
UT = {WOS:000284080500056},
doi = {10.1021/ac102256q},
url = {https://juser.fz-juelich.de/record/11987},
}