% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Hofmann:12388,
      author       = {Hofmann, D. and Wirtz, A. and Santiago-Schübel, B. and
                      Disko, U. and Pohl, M.},
      title        = {{S}tructure elucidation of the thermal degradation products
                      of the nucleotide cofactors {NADH} and {NADPH} by
                      nano-{ESI}-{FTICR}-{MS} and {HPLC}-{MS}},
      journal      = {Analytical and bioanalytical chemistry},
      volume       = {398},
      issn         = {1618-2642},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {PreJuSER-12388},
      year         = {2010},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Redox cofactors like NADH and NADPH are essential for the
                      catalytic activity of several oxidoreductases. Here, we
                      describe a comparative study of the thermal degradation
                      products of both cofactors in the dry and liquid states. The
                      degradation products were first separated, detected, and
                      quantified by high-performance liquid chromatography (HPLC).
                      Subsequently, selected main fractions were investigated by
                      nanoelectrospray ionization-Fourier transform ion cyclotron
                      resonance mass spectrometry (MS). Additionally, HPLC-MS was
                      used to elucidate the structure of all degradation products.
                      From these data, degradation pathways for both the liquid
                      and the solid states were elucidated. Thermal degradation in
                      water is significantly faster compared to degradation in the
                      solid state. Hydrolysis and oxidative ring opening of the
                      reduced nicotinamide adenine dinucleotide (phosphate) were
                      shown to be the main reaction paths. Surprisingly, no
                      significant differences were observed between the
                      degradation of both cofactors in solution and in the solid
                      state. Our results demonstrate that the stability of both
                      cofactors is not limiting at moderate temperatures if they
                      are used in the dry state (e.g., solid/gas catalysis).
                      Significant degradation of dry cofactors was only observed
                      under conditions, which are usually not appropriate for
                      biocatalysis (>95 °C). Besides, the situation is completely
                      different in solution where degradation is already observed
                      at moderate temperatures.},
      keywords     = {Chromatography, High Pressure Liquid: methods / Fourier
                      Analysis / Hot Temperature / NAD: chemistry / NADP:
                      chemistry / Spectrometry, Mass, Electrospray Ionization:
                      methods / NADP (NLM Chemicals) / NAD (NLM Chemicals) / J
                      (WoSType)},
      cin          = {ZCH / IBG-3 / IBT-2 / IBT-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ZCH-20090406 / I:(DE-Juel1)IBG-3-20101118 /
                      I:(DE-Juel1)VDB56 / I:(DE-Juel1)VDB56},
      pnm          = {Terrestrische Umwelt / Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK407 / G:(DE-Juel1)FUEK410},
      shelfmark    = {Biochemical Research Methods / Chemistry, Analytical},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:20803196},
      UT           = {WOS:000284542300006},
      doi          = {10.1007/s00216-010-4111-z},
      url          = {https://juser.fz-juelich.de/record/12388},
}