TY  - JOUR
AU  - Jasnin, M.
AU  - Stadler, A.
AU  - Tehei, M
AU  - Zaccai, G.
TI  - Specific cellular water dynamics observed in vivo by neutron scattering and NMR
JO  - Physical Chemistry Chemical Physics
VL  - 12
SN  - 1463-9076
CY  - Cambridge
PB  - RSC Publ.
M1  - PreJuSER-12506
SP  - 10154 - 10160
PY  - 2010
N1  - Record converted from VDB: 12.11.2012
AB  - Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length (approximately ångstrøm) and time (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid water. It is not "tamed" by confinement. In contrast, in the extreme halophile archaeon, in addition to free and hydration water an intracellular water component was observed with significantly slowed down translational diffusion. The results are discussed and compared to observations in E. coli and Haloarcula marismortui by deuteron spin relaxation in NMR--a method that is sensitive to water rotational dynamics on a wide range of time scales.
KW  - Erythrocytes: metabolism
KW  - Escherichia coli: cytology
KW  - Escherichia coli: metabolism
KW  - Haloarcula marismortui: cytology
KW  - Haloarcula marismortui: metabolism
KW  - Hemoglobins: metabolism
KW  - Humans
KW  - Magnetic Resonance Spectroscopy
KW  - Neutron Diffraction
KW  - Water: metabolism
KW  - Hemoglobins (NLM Chemicals)
KW  - Water (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:20714607
UR  - <Go to ISI:>//WOS:000281352300003
DO  - DOI:10.1039/c0cp01048k
UR  - https://juser.fz-juelich.de/record/12506
ER  -