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@ARTICLE{Jasnin:12506,
author = {Jasnin, M. and Stadler, A. and Tehei, M and Zaccai, G.},
title = {{S}pecific cellular water dynamics observed in vivo by
neutron scattering and {NMR}},
journal = {Physical Chemistry Chemical Physics},
volume = {12},
issn = {1463-9076},
address = {Cambridge},
publisher = {RSC Publ.},
reportid = {PreJuSER-12506},
pages = {10154 - 10160},
year = {2010},
note = {Record converted from VDB: 12.11.2012},
abstract = {Neutron scattering, by using deuterium labelling, revealed
how intracellular water dynamics, measured in vivo in E.
coli, human red blood cells and the extreme halophile,
Haloarcula marismortui, depends on the cell type and nature
of the cytoplasm. The method uniquely permits the
determination of motions on the molecular length
(approximately ångstrøm) and time (pico- to nanosecond)
scales. In the bacterial and human cells, intracellular
water beyond the hydration shells of cytoplasmic
macromolecules and membrane faces flows as freely as liquid
water. It is not "tamed" by confinement. In contrast, in the
extreme halophile archaeon, in addition to free and
hydration water an intracellular water component was
observed with significantly slowed down translational
diffusion. The results are discussed and compared to
observations in E. coli and Haloarcula marismortui by
deuteron spin relaxation in NMR--a method that is sensitive
to water rotational dynamics on a wide range of time
scales.},
keywords = {Erythrocytes: metabolism / Escherichia coli: cytology /
Escherichia coli: metabolism / Haloarcula marismortui:
cytology / Haloarcula marismortui: metabolism / Hemoglobins:
metabolism / Humans / Magnetic Resonance Spectroscopy /
Neutron Diffraction / Water: metabolism / Hemoglobins (NLM
Chemicals) / Water (NLM Chemicals) / J (WoSType)},
cin = {ISB-2},
ddc = {540},
cid = {I:(DE-Juel1)ISB-2-20090406},
pnm = {BioSoft: Makromolekulare Systeme und biologische
Informationsverarbeitung},
pid = {G:(DE-Juel1)FUEK505},
shelfmark = {Chemistry, Physical / Physics, Atomic, Molecular $\&$
Chemical},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:20714607},
UT = {WOS:000281352300003},
doi = {10.1039/c0cp01048k},
url = {https://juser.fz-juelich.de/record/12506},
}