001     12506
005     20200402205929.0
024 7 _ |2 pmid
|a pmid:20714607
024 7 _ |2 DOI
|a 10.1039/C0CP01048K
024 7 _ |2 WOS
|a WOS:000281352300003
024 7 _ |2 ISSN
|a 1463-9076
037 _ _ |a PreJuSER-12506
041 _ _ |a eng
082 _ _ |a 540
084 _ _ |2 WoS
|a Chemistry, Physical
084 _ _ |2 WoS
|a Physics, Atomic, Molecular & Chemical
100 1 _ |a Jasnin, M.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a Specific cellular water dynamics observed in vivo by neutron scattering and NMR
260 _ _ |c 2010
|a Cambridge
|b RSC Publ.
300 _ _ |a 10154 - 10160
336 7 _ |a Journal Article
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336 7 _ |a article
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440 _ 0 |a Physical Chemistry Chemical Physics
|x 1463-9076
|0 4916
|y 35
|v 12
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length (approximately ångstrøm) and time (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid water. It is not "tamed" by confinement. In contrast, in the extreme halophile archaeon, in addition to free and hydration water an intracellular water component was observed with significantly slowed down translational diffusion. The results are discussed and compared to observations in E. coli and Haloarcula marismortui by deuteron spin relaxation in NMR--a method that is sensitive to water rotational dynamics on a wide range of time scales.
536 _ _ |a BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
|c P45
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Erythrocytes: metabolism
650 _ 2 |2 MeSH
|a Escherichia coli: cytology
650 _ 2 |2 MeSH
|a Escherichia coli: metabolism
650 _ 2 |2 MeSH
|a Haloarcula marismortui: cytology
650 _ 2 |2 MeSH
|a Haloarcula marismortui: metabolism
650 _ 2 |2 MeSH
|a Hemoglobins: metabolism
650 _ 2 |2 MeSH
|a Humans
650 _ 2 |2 MeSH
|a Magnetic Resonance Spectroscopy
650 _ 2 |2 MeSH
|a Neutron Diffraction
650 _ 2 |2 MeSH
|a Water: metabolism
650 _ 7 |0 0
|2 NLM Chemicals
|a Hemoglobins
650 _ 7 |0 7732-18-5
|2 NLM Chemicals
|a Water
650 _ 7 |a J
|2 WoSType
700 1 _ |a Stadler, A.
|b 1
|u FZJ
|0 P:(DE-Juel1)VDB86542
700 1 _ |a Tehei, M
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Zaccai, G.
|b 3
|0 P:(DE-HGF)0
773 _ _ |0 PERI:(DE-600)1476244-4
|a 10.1039/c0cp01048k
|g Vol. 12, p. 10154 - 10160
|p 10154 - 10160
|q 12<10154 - 10160
|t Physical Chemistry Chemical Physics
|v 12
|x 1463-9076
|y 2010
856 7 _ |u http://dx.doi.org/10.1039/C0CP01048K
909 C O |o oai:juser.fz-juelich.de:12506
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913 1 _ |k P45
|v BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
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913 2 _ |a DE-HGF
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|l BioSoft Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences
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|v Engineering Cell Function
|x 0
914 1 _ |y 2010
915 _ _ |a JCR/ISI refereed
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|d 31.12.2010
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981 _ _ |a I:(DE-Juel1)ISB-2-20090406


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