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@ARTICLE{Braun:127709,
author = {Braun, Regina and Farré, E. and Schurr, Ulrich and
Matsubara, Shizue},
title = {{E}ffects of light and circulation clock on growth and
chlorophyll accumulation of {N}annochloropsis gaditana
({E}ustigmatophyte)},
journal = {Journal of phycology: a bimonthly of the Phycological
Society of America},
volume = {50},
number = {3},
issn = {0022-3646},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {FZJ-2012-00666},
pages = {515 - 525},
year = {2014},
abstract = {Circadian clocks synchronize various physiological,
metabolic and developmental processes of organisms with
specific phases of recurring changes in their environment
(e.g. day and night or seasons). Here, we investigated
whether the circadian clock plays a role in regulation of
growth and chlorophyll (Chl) accumulation in Nannochloropsis
gaditana, an oleaginous marine microalga which is considered
as a potential feedstock for biofuels and for which a draft
genome sequence has been published. Optical density (OD) of
N. gaditana culture was monitored at 680 and 735 nm under
12:12 h or 18:6 h light-dark (LD) cycles and after switching
to continuous illumination in photobioreactors. In parallel,
Chl fluorescence was measured to assess the quantum yield of
photosystem II. Furthermore, to test if red- or blue-light
photoreceptors are involved in clock entrainment in N.
gaditana, some of the experiments were conducted by using
only red or blue light. Growth and Chl accumulation were
confined to light periods in the LD cycles, increasing more
strongly in the first half than in the second half of the
light periods. After switching to continuous light, rhythmic
oscillations continued (especially for OD680) at least in
the first 24 h, with a $50\%$ decrease in the capacity to
grow and accumulate Chl during the first subjective night.
Pronounced free-running oscillations were induced by blue
light, but not by red light. In contrast, the photosystem II
quantum yield was determined by light conditions. The
results indicate interactions between circadian and light
regulation of growth and Chl accumulation in N. gaditana.},
cin = {IBG-2},
ddc = {570},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {242 - Sustainable Bioproduction (POF2-242) / 89582 - Plant
Science (POF2-89582)},
pid = {G:(DE-HGF)POF2-242 / G:(DE-HGF)POF2-89582},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000337559800010},
doi = {10.1111/jpy.12177},
url = {https://juser.fz-juelich.de/record/127709},
}