TY  - JOUR
AU  - Wesche, Manuel
AU  - Hüske, Martin
AU  - Yakushenko, Alexey
AU  - Brüggemann, Dorothea
AU  - Mayer, Dirk
AU  - Offenhäusser, Andreas
AU  - Wolfrum, Bernhard
TI  - A nanoporous alumina microelectrode array for functional cell–chip coupling
JO  - Nanotechnology
VL  - 23
IS  - 49
SN  - 1361-6528
CY  - Bristol
PB  - IOP Publ.
M1  - FZJ-2013-00346
SP  - 495303 -
PY  - 2012
AB  - The design of electrode interfaces has a strong impact on cell-based bioelectronic applications. We present a new type of microelectrode array chip featuring a nanoporous alumina interface. The chip is fabricated in a combination of top-down and bottom-up processes using state-of-the-art clean room technology and self-assembled generation of nanopores by aluminum anodization. The electrode characteristics are investigated in phosphate buffered saline as well as under cell culture conditions. We show that the modified microelectrodes exhibit decreased impedance compared to planar microelectrodes, which is caused by a nanostructuring effect of the underlying gold during anodization. The stability and biocompatibility of the device are demonstrated by measuring action potentials from cardiomyocyte-like cells growing on top of the chip. Cross sections of the cell–surface interface reveal that the cell membrane seals the nanoporous alumina layer without bending into the sub-50 nm apertures. The nanoporous microelectrode array device may be used as a platform for combining extracellular recording of cell activity with stimulating topographical cues.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000311431400009
DO  - DOI:10.1088/0957-4484/23/49/495303
UR  - https://juser.fz-juelich.de/record/128612
ER  -