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000012874 0247_ $$2DOI$$a10.1016/j.jcrysgro.2010.08.018
000012874 0247_ $$2WOS$$aWOS:000283902800011
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000012874 041__ $$aeng
000012874 082__ $$a540
000012874 084__ $$2WoS$$aCrystallography
000012874 084__ $$2WoS$$aMaterials Science, Multidisciplinary
000012874 084__ $$2WoS$$aPhysics, Applied
000012874 1001_ $$0P:(DE-HGF)0$$aBorshchevskiy, V.$$b0
000012874 245__ $$aIsoprenoid-chained lipid ß-XylOC16+4—A novel molecule for in meso membrane protein crystallization
000012874 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2010
000012874 300__ $$a3326 - 3330
000012874 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000012874 440_0 $$03235$$aJournal of Crystal Growth$$v312$$x0022-0248$$y22
000012874 500__ $$aThis work was financially supported in part by an NEDO International Joint Research Program (Subject: Molecular device for hydrogen production), the AIST Basic Research Program (Subject: Studies on bionanomaterials), the Targeted Proteins Research Program (TPRP) from Ministry of Education, Culture, Sports, Science and Technology of Japan.The work was supported by the program "Chaires d'excellence" edition 2008 of ANR France, CEA(IBS) - HGF(FZJ) STC 5.1 specific agreement and the MC grant for training and career development of researchers (Marie Curie, FP7-PEOPLE-2007-1-1-ITN, project SBMPs) and by an EC FP7 grant for the EDICT consortium (HEALTH-201924). The work was done with the support of Russian State Contracts Nos. 02.740.11.0299, No. 02.740.11.5010, No. P974 of activity 1.2.2 and No. P211 of activity 1.3.2 of the Federal Target Program "Scientific and academic research staff of innovative Russia" for 2009-2013 years. Part of this work was supported by the German Federal Ministry for Education and Research (PhoNa - Photonic Nanomaterials).
000012874 520__ $$aHere we report a successful use of a recently developed isoprenoid-chained lipid family for in meso crystallization of membrane proteins. The isoprenoid-chained lipid 1-O-(3,7,11,15-tetramethylhexadecyl)-beta-D-xyloside (beta-XylOC(16+4)) used as a host lipid for in meso crystallization provided high quality bacteriorhodopsin (bR) crystals (P6(3) space group) diffracting to high resolution and characterized by low twinning ratio. beta-XylOC(16+4) has an isoprenoid chain with methyl branches at each 4th position and a xylose group in the water-soluble part. These peculiarities make the lipid clearly distinguishable in the bR crystalline lattice and provides a unique opportunity to study the role of the host lipid in the in meso crystallization. We conclude that beta-XylOC(16+4) may have a general application for in meso crystallization for a wide range of membrane proteins. The cubic phase of beta-XylOC(16+4) is present over a wide range of temperatures and is stable at low temperature (down to about 8 degrees C). This opens up the possibility of using temperature as a tool for the optimization of in meso crystallization with additional advantages for the crystallization of membrane proteins at lower temperatures where the proteins of interest may be more stable. (C) 2010 Elsevier B.V. All rights reserved.
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000012874 588__ $$aDataset connected to Web of Science
000012874 65320 $$2Author$$aCrystal structure
000012874 65320 $$2Author$$aHigh resolution X-ray diffraction
000012874 65320 $$2Author$$aIn meso crystallization
000012874 65320 $$2Author$$aMembrane proteins
000012874 650_7 $$2WoSType$$aJ
000012874 7001_ $$0P:(DE-HGF)0$$aMoiseeva, E.$$b1
000012874 7001_ $$0P:(DE-HGF)0$$aKuklin, A.$$b2
000012874 7001_ $$0P:(DE-Juel1)131957$$aBüldt, G.$$b3$$uFZJ
000012874 7001_ $$0P:(DE-HGF)0$$aHato, M.$$b4
000012874 7001_ $$0P:(DE-Juel1)VDB482$$aGordeliy, V. I.$$b5$$uFZJ
000012874 773__ $$0PERI:(DE-600)1466514-1$$a10.1016/j.jcrysgro.2010.08.018$$gVol. 312, p. 3326 - 3330$$p3326 - 3330$$q312<3326 - 3330$$tJournal of crystal growth$$v312$$x0022-0248$$y2010
000012874 8567_ $$uhttp://dx.doi.org/10.1016/j.jcrysgro.2010.08.018
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