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@ARTICLE{Borshchevskiy:12877,
      author       = {Borshchevskiy, V. and Efremov, R. and Moiseeva, E. and
                      Büldt, G. and Gordeliy, V. I.},
      title        = {{O}vercoming merohedral twinning in crystals of
                      bacteriorhodopsin grown in lipidic mesophase},
      journal      = {Acta crystallographica / D},
      volume       = {66},
      issn         = {0907-4449},
      address      = {Copenhagen},
      publisher    = {Munksgaard},
      reportid     = {PreJuSER-12877},
      pages        = {26 - 32},
      year         = {2010},
      note         = {The work was supported by the program 'Chaires
                      d'excellence' edition 2008 of ANR France, the
                      CEA(IBS)-HGF(FZJ) STC 5.1 specific agreement and the MC
                      grant for training and career development of researchers
                      (Marie Curie, FP7-PEOPLE-2007-1-1-ITN, project SBMPs). This
                      work was performed in the framework of Russian State
                      Contracts Nos. 02.740.11.0299 (on the subject 'Cell biology
                      and physical properties of membrane proteins' according to
                      claim 'Scientific research by members of Research and
                      Education Center in the field of physico-chemical molecular
                      and cell biology', code 2009-1.1-142-064) and 02.740.11.5010
                      (on the subject 'Investigation of molecular mechanisms of
                      the first step of energy transformation in the cell'
                      according to claim 'Scientific research by groups under the
                      guidance of invited scientists in the field of biology,
                      agricultural science and living system technologies', code
                      2009-1.5-501-002) and a contract P974 on the subject of
                      'Ultrasensitive biosensors on the basis of nanomaterials' in
                      the framework of activity 1.2.2 'Scientific research by
                      scientific groups under the guidance of Candidates of
                      Science' of the Federal Target Program 'Scientific and
                      academic research cadres of innovative Russia' for
                      2009-2013. The authors would like to thank Christian Baeken,
                      Maria Silacheva, Dmitry Bratanov, Yulia Borshchevskaya,
                      Anton Abyzov, Alexandr Shava, Elizaveta Denisenko, Ivan
                      Erofeev and Ivan Gushchin for their help with protein
                      production and crystallization. Eva Pebay-Peyroula and
                      Alexandr Popov are greatly acknowledged for their valuable
                      comments on the manuscript.},
      abstract     = {Twinning is one of the most common crystal-growth defects
                      in protein crystallography. There are neither efficient
                      rational approaches for the growth of nontwinned protein
                      crystals nor are there examples of systematic studies of the
                      dependence of the twinning-ratio distribution on
                      crystallization conditions. The description of the twinning
                      phenomenon has been covered even less for membrane-protein
                      crystals and is non-existent for crystals grown using
                      lipidic phases (in meso). In the present work, possibilities
                      for overcoming merohedral twinning are investigated for
                      crystals of the membrane protein bacteriorhodopsin (bR)
                      grown in meso. It is shown that traditional crystallization
                      additives are not effective in the case of the in meso
                      crystallization of bR. The twinning ratio was determined for
                      310 crystals grown under different crystallization
                      conditions. A correlation of the twinning ratio with the
                      growth rate of the crystals was observed. Slow growth
                      indicated that crystals had a noticeable chance of avoiding
                      twinning. Model calculations were performed in order to
                      rationalize this observation. The calculations confirmed the
                      experimental observation that most crystals consist of two
                      twin domains and showed that under this condition small
                      changes in the probability of twin-domain formation lead to
                      dramatic changes in the number of nontwinned crystals, which
                      explains why slow crystal growth results in a considerable
                      number of nontwinned crystals.},
      keywords     = {Bacteriorhodopsins: chemistry / Bacteriorhodopsins:
                      isolation $\&$ purification / Bacteriorhodopsins: metabolism
                      / Crystallization / Crystallography, X-Ray / Halobacterium
                      salinarum / Lipids: chemistry / Protein Conformation /
                      Protein Structure, Tertiary / Purple Membrane: metabolism /
                      Lipids (NLM Chemicals) / Bacteriorhodopsins (NLM Chemicals)
                      / J (WoSType)},
      cin          = {ISB-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ISB-2-20090406},
      pnm          = {BioSoft: Makromolekulare Systeme und biologische
                      Informationsverarbeitung},
      pid          = {G:(DE-Juel1)FUEK505},
      shelfmark    = {Biochemical Research Methods / Biochemistry $\&$ Molecular
                      Biology / Biophysics / Crystallography},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:20057046},
      UT           = {WOS:000273758800005},
      doi          = {10.1107/S0907444909042838},
      url          = {https://juser.fz-juelich.de/record/12877},
}