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000129029 0247_ $$2doi$$a10.1089/ten.tec.2011.0541
000129029 0247_ $$2pmid$$apmid:22670863
000129029 0247_ $$2ISSN$$a1937-3384
000129029 0247_ $$2ISSN$$a1937-3392
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000129029 037__ $$aFZJ-2013-00552
000129029 041__ $$aeng
000129029 082__ $$a570
000129029 1001_ $$aWalenda, Gudrun$$b0$$eCorresponding author
000129029 245__ $$aHuman platelet lysate gel provides a novel three dimensional-matrix for enhanced culture expansion of mesenchymal stromal cells.
000129029 260__ $$aLarchmont, NY$$bLiebert$$c2012
000129029 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1358779683_4334
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000129029 520__ $$aCell culture in regenerative medicine needs to facilitate efficient expansion according to good manufacturing practice requirements. Human platelet lysate (HPL) can be used as a substitute for fetal calf serum without the risk of xenogeneic immune reactions or transmission of bovine pathogens. Heparin needs to be added as anticoagulant before addition of HPL to culture medium; otherwise, HPL-medium forms a gel within 1 h. Here, we demonstrated that such HPL-gels provide a suitable 3D-matrix for cell culture that-apart from heparin-consists of the same components as the over-layered culture medium. Mesenchymal stromal cells (MSCs) grew in several layers at the interface between HPL-gel and HPL-medium without contact with any artificial biomaterials. Notably, proliferation of MSCs was much higher on HPL-gel compared with tissue culture plastic. Further, the frequency of initial fibroblastoid colony forming units (CFU-f) increased on HPL-gel. The viscous consistency of HPL-gel enabled passaging with a convenient harvesting and reseeding procedure by pipetting cells together with their HPL-matrix-this method does not require washing steps and can easily be automated. The immunophenotype and in vitro differentiation potential toward adipogenic, osteogenic, and chondrogenic lineage were not affected by culture-isolation on HPL-gel. Taken together, HPL-gel has many advantages over conventional plastic surfaces: it facilitates enhanced CFU-f outgrowth, increased proliferation rates, higher cell densities, and nonenzymatic passaging procedures for culture expansion of MSCs.
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000129029 7001_ $$0P:(DE-HGF)0$$aHemeda, Hatim$$b1
000129029 7001_ $$0P:(DE-HGF)0$$aSchneider, Rebekka K$$b2
000129029 7001_ $$0P:(DE-Juel1)128833$$aMerkel, Rudolf$$b3
000129029 7001_ $$0P:(DE-Juel1)128817$$aHoffmann, Bernd$$b4
000129029 7001_ $$0P:(DE-HGF)0$$aWagner, Wolfgang$$b5
000129029 773__ $$0PERI:(DE-600)2401810-7$$a10.1089/ten.tec.2011.0541$$gVol. 18, no. 12, p. 924 - 934$$n12$$p924 - 934$$tTissue engineering / C$$v18$$x1937-3392$$y2012
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000129029 9141_ $$y2012
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