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@ARTICLE{Walenda:129029,
      author       = {Walenda, Gudrun and Hemeda, Hatim and Schneider, Rebekka K
                      and Merkel, Rudolf and Hoffmann, Bernd and Wagner, Wolfgang},
      title        = {{H}uman platelet lysate gel provides a novel three
                      dimensional-matrix for enhanced culture expansion of
                      mesenchymal stromal cells.},
      journal      = {Tissue engineering / C},
      volume       = {18},
      number       = {12},
      issn         = {1937-3392},
      address      = {Larchmont, NY},
      publisher    = {Liebert},
      reportid     = {FZJ-2013-00552},
      pages        = {924 - 934},
      year         = {2012},
      abstract     = {Cell culture in regenerative medicine needs to facilitate
                      efficient expansion according to good manufacturing practice
                      requirements. Human platelet lysate (HPL) can be used as a
                      substitute for fetal calf serum without the risk of
                      xenogeneic immune reactions or transmission of bovine
                      pathogens. Heparin needs to be added as anticoagulant before
                      addition of HPL to culture medium; otherwise, HPL-medium
                      forms a gel within 1 h. Here, we demonstrated that such
                      HPL-gels provide a suitable 3D-matrix for cell culture
                      that-apart from heparin-consists of the same components as
                      the over-layered culture medium. Mesenchymal stromal cells
                      (MSCs) grew in several layers at the interface between
                      HPL-gel and HPL-medium without contact with any artificial
                      biomaterials. Notably, proliferation of MSCs was much higher
                      on HPL-gel compared with tissue culture plastic. Further,
                      the frequency of initial fibroblastoid colony forming units
                      (CFU-f) increased on HPL-gel. The viscous consistency of
                      HPL-gel enabled passaging with a convenient harvesting and
                      reseeding procedure by pipetting cells together with their
                      HPL-matrix-this method does not require washing steps and
                      can easily be automated. The immunophenotype and in vitro
                      differentiation potential toward adipogenic, osteogenic, and
                      chondrogenic lineage were not affected by culture-isolation
                      on HPL-gel. Taken together, HPL-gel has many advantages over
                      conventional plastic surfaces: it facilitates enhanced CFU-f
                      outgrowth, increased proliferation rates, higher cell
                      densities, and nonenzymatic passaging procedures for culture
                      expansion of MSCs.},
      cin          = {ICS-7},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {453 - Physics of the Cell (POF2-453)},
      pid          = {G:(DE-HGF)POF2-453},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:22670863},
      UT           = {WOS:000310782500002},
      doi          = {10.1089/ten.tec.2011.0541},
      url          = {https://juser.fz-juelich.de/record/129029},
}