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@ARTICLE{Walenda:129029,
author = {Walenda, Gudrun and Hemeda, Hatim and Schneider, Rebekka K
and Merkel, Rudolf and Hoffmann, Bernd and Wagner, Wolfgang},
title = {{H}uman platelet lysate gel provides a novel three
dimensional-matrix for enhanced culture expansion of
mesenchymal stromal cells.},
journal = {Tissue engineering / C},
volume = {18},
number = {12},
issn = {1937-3392},
address = {Larchmont, NY},
publisher = {Liebert},
reportid = {FZJ-2013-00552},
pages = {924 - 934},
year = {2012},
abstract = {Cell culture in regenerative medicine needs to facilitate
efficient expansion according to good manufacturing practice
requirements. Human platelet lysate (HPL) can be used as a
substitute for fetal calf serum without the risk of
xenogeneic immune reactions or transmission of bovine
pathogens. Heparin needs to be added as anticoagulant before
addition of HPL to culture medium; otherwise, HPL-medium
forms a gel within 1 h. Here, we demonstrated that such
HPL-gels provide a suitable 3D-matrix for cell culture
that-apart from heparin-consists of the same components as
the over-layered culture medium. Mesenchymal stromal cells
(MSCs) grew in several layers at the interface between
HPL-gel and HPL-medium without contact with any artificial
biomaterials. Notably, proliferation of MSCs was much higher
on HPL-gel compared with tissue culture plastic. Further,
the frequency of initial fibroblastoid colony forming units
(CFU-f) increased on HPL-gel. The viscous consistency of
HPL-gel enabled passaging with a convenient harvesting and
reseeding procedure by pipetting cells together with their
HPL-matrix-this method does not require washing steps and
can easily be automated. The immunophenotype and in vitro
differentiation potential toward adipogenic, osteogenic, and
chondrogenic lineage were not affected by culture-isolation
on HPL-gel. Taken together, HPL-gel has many advantages over
conventional plastic surfaces: it facilitates enhanced CFU-f
outgrowth, increased proliferation rates, higher cell
densities, and nonenzymatic passaging procedures for culture
expansion of MSCs.},
cin = {ICS-7},
ddc = {570},
cid = {I:(DE-Juel1)ICS-7-20110106},
pnm = {453 - Physics of the Cell (POF2-453)},
pid = {G:(DE-HGF)POF2-453},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:22670863},
UT = {WOS:000310782500002},
doi = {10.1089/ten.tec.2011.0541},
url = {https://juser.fz-juelich.de/record/129029},
}