TY  - JOUR
AU  - Cheng, Ji
AU  - Zhu, Geng
AU  - Wu, Lei
AU  - Du, Xiaowei
AU  - Zhang, Huanqian
AU  - Wolfrum, Bernhard
AU  - Jin, Qinghui
AU  - Zhao, Jianlong
AU  - Offenhäusser, Andreas
AU  - Xu, Yuansen
TI  - Photopatterning of self-assembled poly (ethylene) glycol monolayer for neuronal network fabrication
JO  - Journal of neuroscience methods
VL  - 213
IS  - 2
SN  - 0165-0270
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2013-00667
SP  - 196 - 203
PY  - 2013
AB  - The ability to culture individual neurons and direct their connections on functional interfaces provides a platform for investigating information processing in neuronal networks. Numerous methods have been used to design ordered neuronal networks on microelectrode arrays (MEAs) for neuronal electrical activities recording. However, so far, no method has been implemented, which simultaneously provides high-resolution neuronal patterns and low-impedance microelectrode. To achieve this goal, we employed a chemical vapor-deposited, non-fouling poly (ethylene) glycol (PEG) self-assembled monolayer to provide a cell repellant background on the MEAs. Photolithography, together with plasma etching of the PEG monolayer, was used to fabricate different patterns on MEAs. No electrode performance degradation was observed after the whole process. Dissociated cortical neurons were cultured on the modified MEAs, and the patterns were maintained for more than 3 weeks. Spontaneous and evoked neuronal activities were recorded. All of the results demonstrate this surface engineering strategy allows successful patterning of neurons on MEAs, and is useful for future studies of information processing in defined neuronal networks on a chip.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000315557200005
C6  - pmid:23291086
DO  - DOI:10.1016/j.jneumeth.2012.12.020
UR  - https://juser.fz-juelich.de/record/129147
ER  -