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@ARTICLE{Cheng:129147,
author = {Cheng, Ji and Zhu, Geng and Wu, Lei and Du, Xiaowei and
Zhang, Huanqian and Wolfrum, Bernhard and Jin, Qinghui and
Zhao, Jianlong and Offenhäusser, Andreas and Xu, Yuansen},
title = {{P}hotopatterning of self-assembled poly (ethylene) glycol
monolayer for neuronal network fabrication},
journal = {Journal of neuroscience methods},
volume = {213},
number = {2},
issn = {0165-0270},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {FZJ-2013-00667},
pages = {196 - 203},
year = {2013},
abstract = {The ability to culture individual neurons and direct their
connections on functional interfaces provides a platform for
investigating information processing in neuronal networks.
Numerous methods have been used to design ordered neuronal
networks on microelectrode arrays (MEAs) for neuronal
electrical activities recording. However, so far, no method
has been implemented, which simultaneously provides
high-resolution neuronal patterns and low-impedance
microelectrode. To achieve this goal, we employed a chemical
vapor-deposited, non-fouling poly (ethylene) glycol (PEG)
self-assembled monolayer to provide a cell repellant
background on the MEAs. Photolithography, together with
plasma etching of the PEG monolayer, was used to fabricate
different patterns on MEAs. No electrode performance
degradation was observed after the whole process.
Dissociated cortical neurons were cultured on the modified
MEAs, and the patterns were maintained for more than 3
weeks. Spontaneous and evoked neuronal activities were
recorded. All of the results demonstrate this surface
engineering strategy allows successful patterning of neurons
on MEAs, and is useful for future studies of information
processing in defined neuronal networks on a chip.},
cin = {JARA-FIT / PGI-8 / ICS-8},
ddc = {610},
cid = {$I:(DE-82)080009_20140620$ / I:(DE-Juel1)PGI-8-20110106 /
I:(DE-Juel1)ICS-8-20110106},
pnm = {453 - Physics of the Cell (POF2-453) / 423 - Sensorics and
bioinspired systems (POF2-423)},
pid = {G:(DE-HGF)POF2-453 / G:(DE-HGF)POF2-423},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000315557200005},
pubmed = {pmid:23291086},
doi = {10.1016/j.jneumeth.2012.12.020},
url = {https://juser.fz-juelich.de/record/129147},
}