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000001297 1001_ $$0P:(DE-Juel1)VDB55476$$aDebowski, Katharina$$b0$$eCorresponding author$$uFZJ
000001297 245__ $$aIdentifizierung und Charakterisierung eines neuen Bindeproteins für zyklische Nukleotide
000001297 260__ $$aJülich$$bForschungszentrum Jülich GmbH Zentralbibliothek, Verlag$$c2008
000001297 300__ $$aVII, 107 p
000001297 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis
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000001297 4900_ $$0PERI:(DE-600)2414853-2$$832536$$aBerichte des Forschungszentrums Jülich$$v4260$$x0944-2952
000001297 502__ $$aKöln, Univ., Diss., 2007$$bDr. (Univ.)$$cUniv. Köln$$d2007
000001297 500__ $$aRecord converted from VDB: 12.11.2012
000001297 520__ $$aCyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate are important intracellular messengers. Binding of cyclic nucleotides controls the activity of protein kinases, ion channels and guanine-nucleotide-exchange factors in many cells. The SCNBP (soluble cyclic nucleotide-binding protein) is a novel uncharacterized protein predicted to comprise a cyclic nucleotide-binding domain. This protein belongs to neither of the known families of effector proteins for cyclic nucleotides. Within 17 distinct species - from marine invertebrates to humans - genes orthologous to the mouse SCNBP are present. Hence, the SCNBP could belong to a novel class of effector proteins for cyclic nucleotides. Northern blot experiments with mouse tissue indicate that the mRNA of SCNBP is expressed predominantly in the testis and by means of in situ hybridization it was specifically detected in spermatocytes. In the present study, SCNBP expression has been analyzed in mouse testis utilizing specific antibodies. I could provide evidence that two distinct SCNBP variants are present in mouse testis. To approach the physiological function of SCNBP, I identified by immunoprecipitation and mass spectrometry proteins in mouse testis that potentially interact with SCNBP. For a comprehensive biochemical study, SCNBP was heterologously expressed in Chinese hamster ovary (CHO) cells. Following fermentation of these cells in a stirred tank bioreactor I purified SCNBP by affinity chromatography.
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000001297 8564_ $$uhttps://juser.fz-juelich.de/record/1297/files/J%C3%BCl_4260_Debowski.pdf$$yOpenAccess
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000001297 9201_ $$0I:(DE-Juel1)VDB804$$d31.12.2008$$gINB$$kINB-1$$lZelluläre Biophysik$$x1
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