Hauptseite > Publikationsdatenbank > Identifizierung und Charakterisierung eines neuen Bindeproteins für zyklische Nukleotide > print

001     1297
005     20200610184610.0
024 7 _ |2 Handle
|a 2128/16114
037 _ _ |a PreJuSER-1297
088 1 _ |a Juel-4260
088 _ _ |a Juel-4260
|2 JUEL
100 1 _ |0 P:(DE-Juel1)VDB55476
|a Debowski, Katharina
|b 0
|e Corresponding author
|u FZJ
245 _ _ |a Identifizierung und Charakterisierung eines neuen Bindeproteins für zyklische Nukleotide
260 _ _ |a Jülich
|b Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag
|c 2008
300 _ _ |a VII, 107 p
336 7 _ |0 PUB:(DE-HGF)11
|2 PUB:(DE-HGF)
|a Dissertation / PhD Thesis
336 7 _ |0 PUB:(DE-HGF)3
|2 PUB:(DE-HGF)
|a Book
336 7 _ |0 2
|2 EndNote
|a Thesis
336 7 _ |2 DRIVER
|a doctoralThesis
336 7 _ |2 BibTeX
|a PHDTHESIS
336 7 _ |2 DataCite
|a Output Types/Dissertation
336 7 _ |2 ORCID
|a DISSERTATION
490 0 _ |0 PERI:(DE-600)2414853-2
|8 32536
|a Berichte des Forschungszentrums Jülich
|v 4260
|x 0944-2952
500 _ _ |a Record converted from VDB: 12.11.2012
502 _ _ |a Köln, Univ., Diss., 2007
|b Dr. (Univ.)
|c Univ. Köln
|d 2007
520 _ _ |a Cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate are important intracellular messengers. Binding of cyclic nucleotides controls the activity of protein kinases, ion channels and guanine-nucleotide-exchange factors in many cells. The SCNBP (soluble cyclic nucleotide-binding protein) is a novel uncharacterized protein predicted to comprise a cyclic nucleotide-binding domain. This protein belongs to neither of the known families of effector proteins for cyclic nucleotides. Within 17 distinct species - from marine invertebrates to humans - genes orthologous to the mouse SCNBP are present. Hence, the SCNBP could belong to a novel class of effector proteins for cyclic nucleotides. Northern blot experiments with mouse tissue indicate that the mRNA of SCNBP is expressed predominantly in the testis and by means of in situ hybridization it was specifically detected in spermatocytes. In the present study, SCNBP expression has been analyzed in mouse testis utilizing specific antibodies. I could provide evidence that two distinct SCNBP variants are present in mouse testis. To approach the physiological function of SCNBP, I identified by immunoprecipitation and mass spectrometry proteins in mouse testis that potentially interact with SCNBP. For a comprehensive biochemical study, SCNBP was heterologously expressed in Chinese hamster ovary (CHO) cells. Following fermentation of these cells in a stirred tank bioreactor I purified SCNBP by affinity chromatography.
536 _ _ |0 G:(DE-Juel1)FUEK412
|2 G:(DE-HGF)
|a Grundlagen für zukünftige Informationstechnologien
|c P42
|x 0
655 _ 7 |a Hochschulschrift
|x Dissertation (Univ.)
856 4 _ |u https://juser.fz-juelich.de/record/1297/files/J%C3%BCl_4260_Debowski.pdf
|y OpenAccess
909 C O |o oai:juser.fz-juelich.de:1297
|p openaire
|p open_access
|p driver
|p VDB
|p dnbdelivery
913 1 _ |0 G:(DE-Juel1)FUEK412
|b Schlüsseltechnologien
|k P42
|l Grundlagen für zukünftige Informationstechnologien (FIT)
|v Grundlagen für zukünftige Informationstechnologien
|x 0
914 1 _ |y 2008
915 _ _ |0 StatID:(DE-HGF)0510
|2 StatID
|a OpenAccess
920 1 _ |0 I:(DE-Juel1)VDB804
|d 31.12.2008
|g INB
|k INB-1
|l Zelluläre Biophysik
|x 1
970 _ _ |a VDB:(DE-Juel1)102309
980 1 _ |a FullTexts
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a phd
980 _ _ |a I:(DE-Juel1)ICS-4-20110106
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBI-1-20200312
981 _ _ |a I:(DE-Juel1)ICS-4-20110106

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21