% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Tappe:133398,
      author       = {Tappe, Wolfgang and Herbst, Michael and Hofmann, Diana and
                      Köppchen, Stephan and Thiele, Björn and Groeneweg, Joost},
      title        = {{D}egradation of {S}ulfadiazine by {M}icrobacterium lacus
                      {S}train {SDZ}m4, {I}solated from {L}ysimeters {P}reviously
                      {M}anured with {S}lurry from {S}ulfadiazine-{M}edicated
                      {P}igs},
      journal      = {Applied and environmental microbiology},
      volume       = {79},
      number       = {8},
      issn         = {1098-5336},
      address      = {Washington, DC [u.a.]},
      publisher    = {Soc.},
      reportid     = {FZJ-2013-01858},
      pages        = {2572 - 2577},
      year         = {2013},
      abstract     = {Sulfadiazine (SDZ)-degrading bacterial cultures were
                      enriched from the topsoil layer of lysimeters that were
                      formerly treated with manure from pigs medicated with
                      14C-labeled SDZ. The loss of about $35\%$ of the applied
                      radioactivity after an incubation period of 3 years was
                      attributed to CO2 release due to mineralization processes in
                      the lysimeters. Microcosm experiments with moist soil and
                      soil slurries originating from these lysimeters confirmed
                      the presumed mineralization potential, and an SDZ-degrading
                      bacterium was isolated. It was identified as Microbacterium
                      lacus, denoted strain SDZm4. During degradation studies with
                      M. lacus strain SDZm4 using pyrimidine-ring labeled SDZ, SDZ
                      disappeared completely but no 14CO2 was released during 10
                      days of incubation. The entire applied radioactivity (AR)
                      remained in solution and could be assigned to
                      2-aminopyrimidine. In contrast, for parallel incubations but
                      with phenyl ring-labeled SDZ, $56\%$ of the AR was released
                      as 14CO2, $16\%$ was linked to biomass, and $21\%$ remained
                      as dissolved, not yet identified 14C. Thus, it was shown
                      that M. lacus extensively mineralized and partly assimilated
                      the phenyl moiety of the SDZ molecule while forming
                      equimolar amounts of 2-aminopyrimidine. This partial
                      degradation might be an important step in the complete
                      mineralization of SDZ by soil microorganisms.},
      cin          = {IBG-2 / IBG-3},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-2-20101118 / I:(DE-Juel1)IBG-3-20101118},
      pnm          = {245 - Chemicals in the Environment (POF2-245) / 242 -
                      Sustainable Bioproduction (POF2-242)},
      pid          = {G:(DE-HGF)POF2-245 / G:(DE-HGF)POF2-242},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000316956200010},
      pubmed       = {pmid:23396336},
      doi          = {10.1128/AEM.03636-12},
      url          = {https://juser.fz-juelich.de/record/133398},
}