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@ARTICLE{Hoffmann:134962,
author = {Hoffmann, Falk and Strodel, Birgit},
title = {{P}rotein structure prediction using global optimization by
basin-hopping with {NMR} shift restraints},
journal = {The journal of chemical physics},
volume = {138},
number = {025102},
issn = {0021-9606},
address = {Melville, NY},
publisher = {American Institute of Physics},
reportid = {FZJ-2013-02977},
pages = {1-7},
year = {2013},
abstract = {Computational methods that utilize chemical shifts to
produce protein structures at atomic resolution have
recently been introduced. In the current work, we exploit
chemical shifts by combining the basin-hopping approach to
global optimization with chemical shift restraints using a
penalty function. For three peptides, we demonstrate that
this approach allows us to find near-native structures from
fully extended structures within 10 000 basin-hopping
steps. The effect of adding chemical shift restraints is
that the α and β secondary structure elements form within
1000 basin-hopping steps, after which the orientation of the
secondary structure elements, which produces the tertiary
contacts, is driven by the underlying protein force field.
We further show that our chemical shift-restraint BH
approach also works for incomplete chemical shift
assignments, where the information from only one chemical
shift type is considered. For the proper implementation of
chemical shift restraints in the basin-hopping approach, we
determined the optimal weight of the chemical shift penalty
energy with respect to the CHARMM force field in conjunction
with the FACTS solvation model employed in this study. In
order to speed up the local energy minimization procedure,
we developed a function, which continuously decreases the
width of the chemical shift penalty function as the
minimization progresses. We conclude that the basin-hopping
approach with chemical shift restraints is a promising
method for protein structure prediction.},
cin = {ICS-6},
ddc = {540},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {452 - Structural Biology (POF2-452)},
pid = {G:(DE-HGF)POF2-452},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000313642500071},
doi = {10.1063/1.4773406},
url = {https://juser.fz-juelich.de/record/134962},
}