TY  - JOUR
AU  - Komolov, K. E.
AU  - Zinchenko, D. V.
AU  - Churumova, V. A.
AU  - Vaganova, S. A.
AU  - Weiergräber, Oliver H.
AU  - Senin, I. I.
AU  - Philippov, P. P.
AU  - Koch, K. W.
TI  - One of the Ca(2+) binding sites of recoverin exclusively controls interaction with rhodopsin kinase
JO  - Biological chemistry
VL  - 386
SN  - 1431-6730
CY  - Berlin [u.a.]
PB  - de Gruyter
M1  - FZJ-2013-03329
SP  - 285-286
PY  - 2005
AB  - Recoverin is a neuronal calcium sensor protein that controls the activity of rhodopsin kinase in a Ca(2+)-dependent manner. Mutations in the EF-hand Ca2+ binding sites are valuable tools for investigating the functional properties of recoverin. In the recoverin mutant E121Q (Rec E121Q ) the high-affinity Ca2+ binding site is disabled. The non-myristoylated form of Rec E121Q binds one Ca2+ via its second Ca(2+)-binding site (EF-hand 2), whereas the myristoylated variant does not bind Ca2+ at all. Binding of Ca2+ to non-myristoylated Rec E121Q apparently triggers exposure of apolar side chains, allowing for association with hydrophobic matrices. Likewise, an interaction surface for the recoverin target rhodopsin kinase is constituted upon Ca2+ binding to the non-acylated mutant. Structural changes resulting from Ca(2+)-occupation of EF-hand 2 in myristoylated and non-myristoylated recoverin variants are discussed in terms of critical conditions required for biological activity.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000228131400011
DO  - DOI:10.1515/BC.2005.034
UR  - https://juser.fz-juelich.de/record/136533
ER  -