Hauptseite > Publikationsdatenbank > Fetuin-A is a mineral carrier protein: Small angle neutron scattering provides new insight on Fetuin-A controlled calcification inhibition > print

001     13705
005     20240619092021.0
024 7 _ |2 pmid
|a pmid:21156141
024 7 _ |2 pmc
|a pmc:PMC3000477
024 7 _ |2 DOI
|a 10.1016/j.bpj.2010.10.030
024 7 _ |2 WOS
|a WOS:000285438900022
024 7 _ |2 MLZ
|a HeissPJS2010
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037 _ _ |a PreJuSER-13705
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biophysics
100 1 _ |0 P:(DE-HGF)0
|a Heiss, A.
|b 0
245 _ _ |a Fetuin-A is a mineral carrier protein: Small angle neutron scattering provides new insight on Fetuin-A controlled calcification inhibition
260 _ _ |a New York, NY
|b Rockefeller Univ. Press
|c 2010
300 _ _ |a 3986 - 3995
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
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336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |0 882
|a Biophysical Journal
|v 99
|x 0006-3495
|y 12
500 _ _ |a This study was supported by the German Research Foundation (Deutsche Forschungsgemeinschaft) within the priority program "Principles of Biomineralization. A. Heiss thanks Prof. J. Mayer (GFE, Rheinisch-Westfalische Technische Hochschule, Aachen University) for supporting the project.
520 _ _ |a Clinical studies and animal experiments have shown that the serum protein fetuin-A is a highly effective inhibitor of soft tissue calcification. This inhibition mechanism was elucidated on the basis of an in vitro fetuin-A-mineral model system. In a previous study, we found that in a two-stage process ∼100-nm sized calciprotein particles (CPPs) were formed whose final stage was stabilized by a compact outer fetuin-A monolayer against further growth. Quantitative small-angle neutron scattering data analysis revealed that even at a fetuin-A concentration close to the stability limit, only approximately one-half of the mineral ions and only 5% of the fetuin-A were contained in the CPPs. To uncover the interplay of the remaining supersaturated mineral ion fraction and of the 95% non-CPP fetuin-A, we explored the fetuin-A monomer fraction in solution by contrast variation small-angle neutron scattering. Our results suggest that the mineral ions coalesce to subnanometer-sized clusters, reminiscent of Posner clusters, which are stabilized by fetuin-A monomers. Hence, our experiments revealed a second mechanism of long-term mineral ion stabilization by the fetuin-A that is complementary to the formation of CPPs.
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|a Großgeräte für die Forschung mit Photonen, Neutronen und Ionen (PNI)
|c P55
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536 _ _ |0 G:(DE-Juel1)FUEK505
|a BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Calcification, Physiologic
650 _ 2 |2 MeSH
|a Calcium: metabolism
650 _ 2 |2 MeSH
|a Calcium Phosphates: metabolism
650 _ 2 |2 MeSH
|a Carrier Proteins: metabolism
650 _ 2 |2 MeSH
|a Cattle
650 _ 2 |2 MeSH
|a Colloids
650 _ 2 |2 MeSH
|a Minerals: metabolism
650 _ 2 |2 MeSH
|a Neutron Diffraction
650 _ 2 |2 MeSH
|a Protein Binding
650 _ 2 |2 MeSH
|a Scattering, Small Angle
650 _ 2 |2 MeSH
|a Time Factors
650 _ 2 |2 MeSH
|a Ultrafiltration
650 _ 2 |2 MeSH
|a alpha-Fetoproteins: metabolism
650 _ 7 |0 0
|2 NLM Chemicals
|a Calcium Phosphates
650 _ 7 |0 0
|2 NLM Chemicals
|a Carrier Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Colloids
650 _ 7 |0 0
|2 NLM Chemicals
|a Minerals
650 _ 7 |0 0
|2 NLM Chemicals
|a alpha-Fetoproteins
650 _ 7 |0 7440-70-2
|2 NLM Chemicals
|a Calcium
650 _ 7 |2 WoSType
|a J
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|5 EXP:(DE-MLZ)KWS2-20140101
|6 EXP:(DE-MLZ)NL3ao-20140101
|a Forschungs-Neutronenquelle Heinz Maier-Leibnitz
|e KWS-2: Small angle scattering diffractometer
|f NL3ao
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700 1 _ |0 P:(DE-Juel1)VDB4339
|a Pipich, V.
|b 1
|u FZJ
700 1 _ |0 P:(DE-HGF)0
|a Jahnen-Dechent, W.
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700 1 _ |0 P:(DE-Juel1)130962
|a Schwahn, D.
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|a 10.1016/j.bpj.2010.10.030
|g Vol. 99, p. 3986 - 3995
|p 3986 - 3995
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|t Biophysical journal
|v 99
|x 0006-3495
|y 2010
856 7 _ |2 Pubmed Central
|u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3000477
909 C O |o oai:juser.fz-juelich.de:13705
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|b Struktur der Materie
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|l Großgeräteforschung mit Photonen, Neutronen und Ionen
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|a DE-HGF
|b Forschungsbereich Materie
|l In-house research on the structure, dynamics and function of matter
|v Neutrons for Research on Condensed Matter
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914 1 _ |y 2010
915 _ _ |0 StatID:(DE-HGF)0010
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