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@ARTICLE{Qin:13714,
author = {Qin, Z. and Caruso, J.A. and Lai, B. and Matusch, A. and
Becker, J.S.},
title = {{T}race metal imaging with high spatial resolution:
{A}pplications in biomedicine},
journal = {Metallomics},
volume = {3},
issn = {1756-5901},
address = {Cambridge},
publisher = {RSC Publ.},
reportid = {PreJuSER-13714},
pages = {28 - 37},
year = {2011},
note = {This work was supported by an AHA National Scientist
Development Grant (0835268N).},
abstract = {New generations of analytical techniques for imaging of
metals are pushing hitherto boundaries of spatial resolution
and quantitative analysis in biology. Because of this, the
application of these imaging techniques described herein to
the study of the organization and dynamics of metal cations
and metal-containing biomolecules in biological cell and
tissue is becoming an important issue in biomedical
research. In the current review, three common metal imaging
techniques in biomedical research are introduced, including
synchrotron X-ray fluorescence (SXRF) microscopy, secondary
ion mass spectrometry (SIMS), and laser ablation inductively
coupled plasma mass spectrometry (LA-ICP-MS). These are
exemplified by a demonstration of the dopamine-Fe complexes,
by assessment of boron distribution in a boron neutron
capture therapy cell model, by mapping Cu and Zn in human
brain cancer and a rat brain tumor model, and by the
analysis of metal topography within neuromelanin. These
studies have provided solid evidence that demonstrates that
the sensitivity, spatial resolution, specificity, and
quantification ability of metal imaging techniques is
suitable and highly desirable for biomedical research.
Moreover, these novel studies on the nanometre scale (e.g.,
of individual single cells or cell organelles) will lead to
a better understanding of metal processes in cells and
tissues.},
keywords = {Animals / Biomedical Technology: methods / Brain: cytology
/ Brain: metabolism / Brain: pathology / Copper: analysis /
Copper: chemistry / Cytological Techniques: methods /
Glioblastoma: metabolism / Glioblastoma: pathology / Humans
/ Mass Spectrometry / Molecular Imaging: methods / Rats /
Spectrometry, X-Ray Emission / Zinc: analysis / Zinc:
chemistry / Copper (NLM Chemicals) / Zinc (NLM Chemicals) /
J (WoSType)},
cin = {ZCH / INM-2},
ddc = {690},
cid = {I:(DE-Juel1)ZCH-20090406 / I:(DE-Juel1)INM-2-20090406},
pnm = {Funktion und Dysfunktion des Nervensystems (FUEK409) /
89571 - Connectivity and Activity (POF2-89571)},
pid = {G:(DE-Juel1)FUEK409 / G:(DE-HGF)POF2-89571},
shelfmark = {Biochemistry $\&$ Molecular Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21140012},
UT = {WOS:000286057100002},
doi = {10.1039/c0mt00048e},
url = {https://juser.fz-juelich.de/record/13714},
}