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000137714 1001_ $$0P:(DE-HGF)0$$aJanoštiak, Radoslav$$b0$$eCorresponding author
000137714 245__ $$aCAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics
000137714 260__ $$aBasel$$bBirkhäuser$$c2014
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000137714 520__ $$aFocal adhesions are cellular structures through which both mechanical forces and regulatory signals are transmitted. Two focal adhesion-associated proteins, Crk-associated substrate (CAS) and vinculin, were both independently shown to be crucial for the ability of cells to transmit mechanical forces and to regulate cytoskeletal tension. Here, we identify a novel, direct binding interaction between CAS and vinculin. This interaction is mediated by the CAS SRC homology 3 domain and a proline-rich sequence in the hinge region of vinculin. We show that CAS localization in focal adhesions is partially dependent on vinculin, and that CAS–vinculin coupling is required for stretch-induced activation of CAS at the Y410 phosphorylation site. Moreover, CAS–vinculin binding significantly affects the dynamics of CAS and vinculin within focal adhesions as well as the size of focal adhesions. Finally, disruption of CAS binding to vinculin reduces cell stiffness and traction force generation. Taken together, these findings strongly implicate a crucial role of CAS–vinculin interaction in mechanosensing and focal adhesion dynamics
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000137714 7001_ $$0P:(DE-HGF)0$$aBrábek, Jan$$b1
000137714 7001_ $$0P:(DE-HGF)0$$aAuernheimer, Vera$$b2
000137714 7001_ $$0P:(DE-HGF)0$$aTatárová, Zuzana$$b3
000137714 7001_ $$0P:(DE-HGF)0$$aLautscham, Lena A.$$b4
000137714 7001_ $$0P:(DE-HGF)0$$aDey, Tuli$$b5
000137714 7001_ $$0P:(DE-HGF)0$$aGemperle, Jakub$$b6
000137714 7001_ $$0P:(DE-Juel1)128833$$aMerkel, Rudolf$$b7$$ufzj
000137714 7001_ $$0P:(DE-HGF)0$$aGoldmann, Wolfgang H.$$b8
000137714 7001_ $$0P:(DE-HGF)0$$aFabry, Ben$$b9
000137714 7001_ $$0P:(DE-HGF)0$$aRösel, Daniel$$b10
000137714 773__ $$0PERI:(DE-600)1458497-9$$a10.1007/s00018-013-1450-x$$n4$$p727-744$$tCellular and molecular life sciences$$v71$$x1420-9071
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000137714 9141_ $$y2014
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