%0 Journal Article
%A Yakushenko, Alexey
%A Kätelhön, Enno
%A Wolfrum, Bernhard
%T Parallel On-Chip Analysis of Single Vesicle Neurotransmitter Release
%J Analytical chemistry
%V 85
%N 11
%@ 1520-6882
%C Columbus, Ohio
%I American Chemical Society
%M FZJ-2013-04547
%P 5483 - 5490
%D 2013
%X Real-time investigations of neurotransmitter release provide a direct insight on the mechanisms involved in synaptic communication. Carbon fiber microelectrodes are state-of-the-art tools for electrochemical measurements of single vesicle neurotransmitter release. Yet, they lack high-throughput capabilities that are required for collecting robust statistically significant data across multiple samples. Here, we present a chip-based recording system enabling parallel in vitro measurements of individual neurotransmitter release events from cells, cultured directly on planar multielectrode arrays. The applicability of this cell-based platform to pharmacological screening is demonstrated by resolving minute concentration-dependent effects of the dopamine reuptake inhibitor nomifensine on recorded single-vesicle release events from PC12 cells. The experimental results, showing an increased half-time of the recorded events, are complemented by an analytical model for the verification of drug action.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000320096900030
%$ pmid:23642073
%R 10.1021/ac4006183
%U https://juser.fz-juelich.de/record/138416