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000138416 1001_ $$0P:(DE-Juel1)138367$$aYakushenko, Alexey$$b0$$eCorresponding author$$ufzj
000138416 245__ $$aParallel On-Chip Analysis of Single Vesicle Neurotransmitter Release
000138416 260__ $$aColumbus, Ohio$$bAmerican Chemical Society$$c2013
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000138416 520__ $$aReal-time investigations of neurotransmitter release provide a direct insight on the mechanisms involved in synaptic communication. Carbon fiber microelectrodes are state-of-the-art tools for electrochemical measurements of single vesicle neurotransmitter release. Yet, they lack high-throughput capabilities that are required for collecting robust statistically significant data across multiple samples. Here, we present a chip-based recording system enabling parallel in vitro measurements of individual neurotransmitter release events from cells, cultured directly on planar multielectrode arrays. The applicability of this cell-based platform to pharmacological screening is demonstrated by resolving minute concentration-dependent effects of the dopamine reuptake inhibitor nomifensine on recorded single-vesicle release events from PC12 cells. The experimental results, showing an increased half-time of the recorded events, are complemented by an analytical model for the verification of drug action.
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000138416 7001_ $$0P:(DE-Juel1)128700$$aKätelhön, Enno$$b1$$ufzj
000138416 7001_ $$0P:(DE-Juel1)128745$$aWolfrum, Bernhard$$b2$$ufzj
000138416 773__ $$0PERI:(DE-600)1483443-1$$a10.1021/ac4006183$$gVol. 85, no. 11, p. 5483 - 5490$$n11$$p5483 - 5490$$tAnalytical chemistry$$v85$$x1520-6882$$y2013
000138416 8564_ $$zPublished final document.
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