TY  - JOUR
AU  - Yakushenko, Alexey
AU  - Kätelhön, Enno
AU  - Wolfrum, Bernhard
TI  - Parallel On-Chip Analysis of Single Vesicle Neurotransmitter Release
JO  - Analytical chemistry
VL  - 85
IS  - 11
SN  - 1520-6882
CY  - Columbus, Ohio
PB  - American Chemical Society
M1  - FZJ-2013-04547
SP  - 5483 - 5490
PY  - 2013
AB  - Real-time investigations of neurotransmitter release provide a direct insight on the mechanisms involved in synaptic communication. Carbon fiber microelectrodes are state-of-the-art tools for electrochemical measurements of single vesicle neurotransmitter release. Yet, they lack high-throughput capabilities that are required for collecting robust statistically significant data across multiple samples. Here, we present a chip-based recording system enabling parallel in vitro measurements of individual neurotransmitter release events from cells, cultured directly on planar multielectrode arrays. The applicability of this cell-based platform to pharmacological screening is demonstrated by resolving minute concentration-dependent effects of the dopamine reuptake inhibitor nomifensine on recorded single-vesicle release events from PC12 cells. The experimental results, showing an increased half-time of the recorded events, are complemented by an analytical model for the verification of drug action.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000320096900030
C6  - pmid:23642073
DO  - DOI:10.1021/ac4006183
UR  - https://juser.fz-juelich.de/record/138416
ER  -