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001     138416
005     20240619091101.0
024 7 _ |a 10.1021/ac4006183
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024 7 _ |a 1520-6882
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037 _ _ |a FZJ-2013-04547
082 _ _ |a 540
100 1 _ |a Yakushenko, Alexey
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245 _ _ |a Parallel On-Chip Analysis of Single Vesicle Neurotransmitter Release
260 _ _ |a Columbus, Ohio
|c 2013
|b American Chemical Society
336 7 _ |a Journal Article
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500 _ _ |3 POF3_Assignment on 2016-02-29
520 _ _ |a Real-time investigations of neurotransmitter release provide a direct insight on the mechanisms involved in synaptic communication. Carbon fiber microelectrodes are state-of-the-art tools for electrochemical measurements of single vesicle neurotransmitter release. Yet, they lack high-throughput capabilities that are required for collecting robust statistically significant data across multiple samples. Here, we present a chip-based recording system enabling parallel in vitro measurements of individual neurotransmitter release events from cells, cultured directly on planar multielectrode arrays. The applicability of this cell-based platform to pharmacological screening is demonstrated by resolving minute concentration-dependent effects of the dopamine reuptake inhibitor nomifensine on recorded single-vesicle release events from PC12 cells. The experimental results, showing an increased half-time of the recorded events, are complemented by an analytical model for the verification of drug action.
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700 1 _ |a Kätelhön, Enno
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700 1 _ |a Wolfrum, Bernhard
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773 _ _ |a 10.1021/ac4006183
|g Vol. 85, no. 11, p. 5483 - 5490
|p 5483 - 5490
|n 11
|0 PERI:(DE-600)1483443-1
|t Analytical chemistry
|v 85
|y 2013
|x 1520-6882
856 4 _ |z Published final document.
856 4 _ |u https://juser.fz-juelich.de/record/138416/files/FZJ-2013-04547_PV.pdf
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