TY  - JOUR
AU  - Yakushenko, Alexey
AU  - Gong, Zheng
AU  - Maybeck, Vanessa
AU  - Hofmann, Boris
AU  - Gu, Erdan
AU  - Dawson, Martin
AU  - Offenhäusser, Andreas
AU  - Wolfrum, Bernhard
TI  - On-chip optical stimulation and electrical recording from cells
JO  - Journal of biomedical optics
VL  - 18
IS  - 11
SN  - 1083-3668
CY  - Bellingham, Wash.
PB  - SPIE
M1  - FZJ-2013-04552
SP  - 111402 
PY  - 2013
AB  - We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip for short- and long-term electrophysiological experiments. To show the functionality of the device, we transfected a cardiomyocyte-like cell line (HL-1) with a light-sensitive protein channelrhodopsin. We monitored action potentials of individual, spontaneously beating, HL-1 cells growing on the chip by extracellular electrical recordings. On-chip optical stimulation was demonstrated by triggering network activity in a confluent HL-1 cell culture and visualized by calcium imaging. We see the potential of our system for electrophysiological experiments with optogenetically modified cells. Optical stimulation can be performed directly on the chip without additional optical components or external light sources.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000328895100007
C6  - pmid:23788259
DO  - DOI:10.1117/1.JBO.18.11.111402
UR  - https://juser.fz-juelich.de/record/138421
ER  -