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@ARTICLE{Babcock:139042,
author = {Babcock, E. and Salhi, Z. and Appavou, M. -S. and
Feoktystov, A. and Pipich, V. and Radulescu, A. and Ossovyi,
V. and Staringer, S. and Ioffe, A.},
title = {{P}olarization {A}nalysis with 3{H}e {S}pin {F}ilters for
{S}eparating {C}oherent from {I}ncoherent {S}cattering in
{S}oft {M}atter {S}tudies},
journal = {Physics procedia},
volume = {42},
issn = {1875-3892},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {FZJ-2013-05063},
pages = {154 - 162},
year = {2013},
abstract = {In soft matter small angle neutron scattering (SANS)
studies at large Q values, incoherent scattering becomes the
dominant signal. In the Q-range of interest to this work,
from 0.2 Å−1 to about 1.0 Å−1, the coherent scattering
from the typical protein or polymer in a D2O buffer solution
inevitably drops one to two orders of magnitude or more
below the total scattering. Even after careful and accurate
subtraction of the measured D2O buffer scattering, the
remaining corrected, i.e. sample-only, signal will still be
dominated by diffuse incoherent scattering from hydrogen in
the sample itself. This is the exact region of interest when
one wishes to probe the structural changes in “living”
proteins caused by interactions and motions related to
function. To further complicate the problem, there is strong
motivation to measure this Q-regime at very low
concentrations because it has been shown with wide angle
X-ray scattering that proteins can undergo
concentration-dependent structural changes that rapidly
increase below concentrations of about $5\%$ [1] motivating
the study of protein solutions at ever lower concentrations.
In this case the signal from the protein will inevitably
become much less than the scattering of the D2O buffer
solution it is contained in. Polarization analysis offers
the opportunity to separate the weak coherent signal from
the larger incoherent signal and perhaps enable measurements
under the conditions described above. This paper will
address the issues associated with the correct separation of
coherent and incoherent scattering for soft matter samples.
We have performed tests measurements on KWS2 which show the
viability of the method on a protonated α-lactalbumin
solution at $2.5\%$ (1 mm thick) and $0.25\%$ (2 mm thick)
concentrations in a D2O buffer solution. Additionally
describe a the method of implementation using 3He spin
filters, some practical considerations, and future plans for
a dedicated device at the JCNS.},
cin = {PGI-4 / Neutronenstreuung ; JCNS-1 / JCNS-2 / JCNS
(München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / JARA-FIT / ICS-1},
ddc = {530},
cid = {I:(DE-Juel1)PGI-4-20110106 / I:(DE-Juel1)JCNS-1-20110106 /
I:(DE-Juel1)JCNS-2-20110106 /
I:(DE-Juel1)JCNS-FRM-II-20110218 /
$I:(DE-82)080009_20140620$ / I:(DE-Juel1)ICS-1-20110106},
pnm = {422 - Spin-based and quantum information (POF2-422) / 424 -
Exploratory materials and phenomena (POF2-424) / 542 -
Neutrons (POF2-542) / 544 - In-house Research with PNI
(POF2-544) / 54G - JCNS (POF2-54G24) / 451 - Soft Matter
Composites (POF2-451)},
pid = {G:(DE-HGF)POF2-422 / G:(DE-HGF)POF2-424 /
G:(DE-HGF)POF2-542 / G:(DE-HGF)POF2-544 /
G:(DE-HGF)POF2-54G24 / G:(DE-HGF)POF2-451},
experiment = {EXP:(DE-MLZ)KWS2-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000331183800024},
doi = {10.1016/j.phpro.2013.03.190},
url = {https://juser.fz-juelich.de/record/139042},
}