TY  - JOUR
AU  - Bock, Lars V
AU  - Blau, Christian
AU  - Schröder, Gunnar
AU  - Davydov, Iakov I
AU  - Fischer, Niels
AU  - Stark, Holger
AU  - Rodnina, Marina V
AU  - Vaiana, Andrea C
AU  - Grubmüller, Helmut
TI  - Energy barriers and driving forces in tRNA translocation through the ribosome
JO  - Nature structural & molecular biology
VL  - 2690
SN  - 1545-9993
CY  - London [u.a.]
PB  - Nature Publishing Group
M1  - FZJ-2013-05183
SP  - 1-8
PY  - 2013
AB  - During protein synthesis, tRNAs move from the ribosome's aminoacyl to peptidyl to exit sites. Here we investigate conformational motions during spontaneous translocation, using molecular dynamics simulations of 13 intermediate-translocation-state models obtained by combining Escherichia coli ribosome crystal structures with cryo-EM data. Resolving fast transitions between states, we find that tRNA motions govern the transition rates within the pre- and post-translocation states. Intersubunit rotations and L1-stalk motion exhibit fast intrinsic submicrosecond dynamics. The L1 stalk drives the tRNA from the peptidyl site and links intersubunit rotation to translocation. Displacement of tRNAs is controlled by 'sliding' and 'stepping' mechanisms involving conserved L16, L5 and L1 residues, thus ensuring binding to the ribosome despite large-scale tRNA movement. Our results complement structural data with a time axis, intrinsic transition rates and molecular forces, revealing correlated functional motions inaccessible by other means.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000328007600011
C6  - pmid:24186064
DO  - DOI:10.1038/nsmb.2690
UR  - https://juser.fz-juelich.de/record/139180
ER  -