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@INPROCEEDINGS{Schrader:139570,
author = {Schrader, Tobias and Ostermann, Andreas and Monkenbusch,
Michael and Laatsch, Bernhard and Jüttner, Philipp and
Petry, Winfried and Richter, Dieter},
title = {{F}irst {R}esults from {M}easurements at the {N}ew{N}eutron
{D}iffractometer “{B}io{D}iff”},
reportid = {FZJ-2013-05553},
year = {2013},
abstract = {The neutron diffractometer BioDiff is a joint project
between the Forschungszentrum Jülich (FZJ/JCNS) and the
Forschungs-Neutronenquelle Heinz Maier-Leibnitz (FRM II).
The instrument BioDiff is especially designed to collect
data from crystals with large unit cells. The main field of
application is the structure analysis of proteins,
especially the determination of hydrogen atom positions.
Typical scientific questions addressed are the determination
of protonation states of amino acid side chains and the
characterization of the hydrogen bonding networks between
the protein and an inhibitor or substrate. The orientation
of water molecules in the active centre of the protein can
also be determined (c. f. Fig. 1). The main advantage of the
monochromatic instrument BioDiff is the possibility to adapt
the wavelength between 2.4 Å and 5.6 Å to obtain a
compromise between higher scattering yields at longer
wavelengths and better resolution at smaller wavelengths. In
this contribution the most recent results from the
instrument BioDiff will be presented concentrating on the
recently published measurements on the Toho-1 -lactamase
mutant R274N/R276N [1] and on myoglobin crystals [2]. The
latter example will be given because it shows that data from
BioDiff can be compared to similar instruments e. g. the
BIX-3 in Japan. Here, the water network around the outer
surface of the protein was investigated. Figure 1 nicely
shows how the orientation of water molecules can be
determined on the surface of the protein if the water
molecules are fixed well enough due to a surrounding
hydrogen bonding network.},
month = {Aug},
date = {2013-08-25},
organization = {The 28th Meeting of the European
Crystallographic Association, Warwick
(United Kingdom), 25 Aug 2013 - 29 Aug
2013},
cin = {JCNS-1 / JCNS (München) ; Jülich Centre for Neutron
Science JCNS (München) ; JCNS-FRM-II / ICS-1},
cid = {I:(DE-Juel1)JCNS-1-20110106 /
I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)ICS-1-20110106},
pnm = {451 - Soft Matter Composites (POF2-451) / 54G - JCNS
(POF2-54G24)},
pid = {G:(DE-HGF)POF2-451 / G:(DE-HGF)POF2-54G24},
typ = {PUB:(DE-HGF)24},
url = {https://juser.fz-juelich.de/record/139570},
}